biostudies-literatureUnknown80(4)Tellapragada CScilife Pandemic Laboratory Preparedness<h4>Objectives</h4>We aimed to investigate the in vitro activity and genetic determinants of decreased susceptibility (DS; MIC > 4 mg/L) to cefepime/zidebactam of carbapenemase-producing Escherichia coli.<h4>Methods</h4>Clinical isolates (N = 150) of carbapenemase-producing E. coli (CP-EC) belonging to seven distinct STs, isolated at a university clinical microbiology laboratory during 2019-2023 in Stockholm, Sweden were included. MICs for cefepime/zidebactam were determined using the broth microdilution method and interpreted using the tentative EUCAST clinical breakpoints (Susceptible; MIC < 4 mg/L; based on cefepime breakpoint). Whole genome sequences of the isolates were analysed with an emphasis on identifying alterations in PBPs 2 and 3.<h4>Results</h4>Of the 150 isolates, 145 (96.6%The Journal of antimicrobial chemotherapy1137-1140https://www.ebi.ac.uk/biostudies/studies/S-EPMC11962383biostudies-literatureDecreased susceptibility to cefepime/zidebactam among carbapenemase-producing Escherichia coli from Stockholm, Sweden with alterations in PBP2.PMC11962383Dunleavy CJonsson PGiske CGTellapragada CfalseDecreased susceptibility to cefepime/zidebactam among carbapenemase-producing Escherichia coli from Stockholm, Sweden with alterations in PBP2.<h4>Objectives</h4>We aimed to investigate the in vitro activity and genetic determinants of decreased susceptibility (DS; MIC > 4 mg/L) to cefepime/zidebactam of carbapenemase-producing Escherichia coli.<h4>Methods</h4>Clinical isolates (N = 150) of carbapenemase-producing E. coli (CP-EC) belonging to seven distinct STs, isolated at a university clinical microbiology laboratory during 2019-2023 in Stockholm, Sweden were included. MICs for cefepime/zidebactam were determined using the broth microdilution method and interpreted using the tentative EUCAST clinical breakpoints (Susceptible; MIC < 4 mg/L; based on cefepime breakpoint). Whole genome sequences of the isolates were analysed with an emphasis on identifying alterations in PBPs 2 and 3.<h4>Results</h4>Of the 150 isolates, 145 (96.6%2025-01-01T00:00:00Z2025 Apr2025-07-11T03:04:08.248Z2025-07-11T03:04:08.248ZS-EPMC119623833996009110.1093/jac/dkaf045