{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Greenman AC"],"funding":["NHLBI NIH HHS"],"pagination":["82-92"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11963209"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["200"],"pubmed_abstract":["Cardiac myosin binding protein-C (cMyBP-C) is a sarcomere regulatory protein consisting of 11 well-folded immunoglobulin-like (Ig-like) and fibronectin type-III domains with the individual domains numbered C0-C10. Despite progress in understanding the functions of the N' and C'-terminal ends of the protein, our understanding of the functional effects of the middle domains (C3-C4-C5-C6-C7) is still limited. Here we aimed to determine the functional significance of the middle domains by replacing endogenous cMyBP-C with recombinant proteins with and without the middle domains using our \"cut and paste\" SpyC3 mouse model. Specifically, we deleted domains C3-C7 or substituted these domains with unrelated Ig-like domains from titin to behave as inert \"spacer\" domains. Replacement with the spacer"],"journal":["Journal of molecular and cellular cardiology"],"pubmed_title":["Autoinhibition of cMyBP-C by its middle domains."],"pmcid":["PMC11963209"],"funding_grant_id":["F32 HL170509","R01 HL140925","R01 HL080367"],"pubmed_authors":["Greenman AC","Harris SP","Sadler RL"],"additional_accession":[]},"is_claimable":false,"name":"Autoinhibition of cMyBP-C by its middle domains.","description":"Cardiac myosin binding protein-C (cMyBP-C) is a sarcomere regulatory protein consisting of 11 well-folded immunoglobulin-like (Ig-like) and fibronectin type-III domains with the individual domains numbered C0-C10. Despite progress in understanding the functions of the N' and C'-terminal ends of the protein, our understanding of the functional effects of the middle domains (C3-C4-C5-C6-C7) is still limited. Here we aimed to determine the functional significance of the middle domains by replacing endogenous cMyBP-C with recombinant proteins with and without the middle domains using our \"cut and paste\" SpyC3 mouse model. Specifically, we deleted domains C3-C7 or substituted these domains with unrelated Ig-like domains from titin to behave as inert \"spacer\" domains. Replacement with the spacer","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-07-16T23:19:57.914Z","creation":"2026-07-12T03:11:10.428Z"},"accession":"S-EPMC11963209","cross_references":{"pubmed":["39923987"],"doi":["10.1016/j.yjmcc.2025.02.002"]}}