{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Feng Z"],"funding":["Natural Science Foundation of Fujian Province","National Natural Science Foundation of China"],"pagination":["136"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11965083"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(1)"],"pubmed_abstract":["Porcine epidemic diarrhea virus (PEDV) has caused significant losses in the pork industry, but the mechanism of PEDV infection is still unclear. On the basis of our RNA-Seq data and due to the potential role of sialic acid as a coreceptor, we investigated the function of sialic acid-binding Ig-like lectin 15 (Siglec-15) to determine its role as a receptor in PEDV infection. We found that Siglec-15 enhances PEDV infection by promoting viral adsorption to host cells. Coimmunoprecipitation and immunofluorescence assays revealed that Siglec-15 binds to the S1 subunit and M protein of PEDV. PEDV infectivity was significantly reduced in Siglec-15 knockout mice. In addition, we developed a monoclonal antibody targeting Siglec-15 that can effectively inhibit PEDV infection both in vitro and in viv"],"journal":["Cellular and molecular life sciences : CMLS"],"pubmed_title":["Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection."],"pmcid":["PMC11965083"],"funding_grant_id":["82350126","82171991","2022J01652"],"pubmed_authors":["Chen Q","Feng Z","Chen J","Fu Y","Li Z","Huang W","He X","Chen Y","Liu C","Shen Y","Yang S","Lin M","Zhao H"],"additional_accession":[]},"is_claimable":false,"name":"Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection.","description":"Porcine epidemic diarrhea virus (PEDV) has caused significant losses in the pork industry, but the mechanism of PEDV infection is still unclear. On the basis of our RNA-Seq data and due to the potential role of sialic acid as a coreceptor, we investigated the function of sialic acid-binding Ig-like lectin 15 (Siglec-15) to determine its role as a receptor in PEDV infection. We found that Siglec-15 enhances PEDV infection by promoting viral adsorption to host cells. Coimmunoprecipitation and immunofluorescence assays revealed that Siglec-15 binds to the S1 subunit and M protein of PEDV. PEDV infectivity was significantly reduced in Siglec-15 knockout mice. In addition, we developed a monoclonal antibody targeting Siglec-15 that can effectively inhibit PEDV infection both in vitro and in viv","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-06-01T05:40:33.823Z","creation":"2025-07-12T03:04:21.237Z"},"accession":"S-EPMC11965083","cross_references":{"pubmed":["40172660"],"doi":["10.1007/s00018-025-05672-2"]}}