biostudies-literatureUnknown15(1)Mercanoglu BUniversitätsklinikum Hamburg-Eppendorf (UKE)Altered O-glycosylation is a key contributor to various pathophysiological processes. Notably, the expression of the Tn antigen is primarily attributed to dysfunction of the chaperone Cosmc, while the overexpression of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) has also been implicated in numerous diseases. We generated a transgenic mouse model with conditional Cosmc-knockout and simultaneous overexpression of polypeptide N-acetylgalactosaminyltransferase 2 (GalNT2) mediated by the pancreas-specific transcription factor 1a (Ptf1a)-Cre mouse strain to investigate the effect of Tn antigen overexpression on the pancreas in vivo. Histopathological examination of the transgenic pancreas revealed a chronic pancreatitis phenotype with interlobular fibrosis and focal necrosis afterScientific reports11306https://www.ebi.ac.uk/biostudies/studies/S-EPMC11965491biostudies-literatureOverexpression of Tn antigen induces chronic pancreatitis in mice.PMC11965491Wolters-Eisfeld GNeuburg EKempski JBockhorn MMercanoglu BSchraps NGiannou ADWagener CMelling NHackert TfalseOverexpression of Tn antigen induces chronic pancreatitis in mice.Altered O-glycosylation is a key contributor to various pathophysiological processes. Notably, the expression of the Tn antigen is primarily attributed to dysfunction of the chaperone Cosmc, while the overexpression of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) has also been implicated in numerous diseases. We generated a transgenic mouse model with conditional Cosmc-knockout and simultaneous overexpression of polypeptide N-acetylgalactosaminyltransferase 2 (GalNT2) mediated by the pancreas-specific transcription factor 1a (Ptf1a)-Cre mouse strain to investigate the effect of Tn antigen overexpression on the pancreas in vivo. Histopathological examination of the transgenic pancreas revealed a chronic pancreatitis phenotype with interlobular fibrosis and focal necrosis after2025-01-01T00:00:00Z2025 Apr2025-07-14T03:03:05.434Z2025-07-14T03:03:05.434ZS-EPMC119654914017568910.1038/s41598-025-96060-0