biostudies-literatureUnknown16Huang Y<h4>Introduction</h4><i>Blechnopsis orientalis</i> (L.) C. Presl is a medicinal and edible fern species belonging to the Blechnaceae family. Currently, the complete mitochondrial genome of <i>B. orientalis</i>, as well as those of other Blechnaceae species, remains unreported, and studies on fern mitochondrial genome are limited.<h4>Methods</h4>In this study, the <i>B. orientalis</i> mitochondrial genome was sequenced using both Nanopore PromethION and Illumina NovaSeq 6000 platforms. Genome annotation was performed using MITOFY and MFANNOT, with structural visualization via OGDRAW. In-depth analyses were conducted, including assessments of non-synonymous/synonymous mutation ratios (Ka/Ks), codon usage bias, repeat sequence identification, RNA editing site prediction, collinearity, and theFrontiers in plant science1534171https://www.ebi.ac.uk/biostudies/studies/S-EPMC11965612biostudies-literatureThe first complete mitochondrial genome assembly and comparative analysis of the fern Blechnaceae family: &lt;i&gt;Blechnopsis orientalis&lt;/i&gt;.PMC11965612Xu LMen WYang YHuang YXing YKang TDou DXue HHou WfalseThe first complete mitochondrial genome assembly and comparative analysis of the fern Blechnaceae family: &lt;i&gt;Blechnopsis orientalis&lt;/i&gt;.<h4>Introduction</h4><i>Blechnopsis orientalis</i> (L.) C. Presl is a medicinal and edible fern species belonging to the Blechnaceae family. Currently, the complete mitochondrial genome of <i>B. orientalis</i>, as well as those of other Blechnaceae species, remains unreported, and studies on fern mitochondrial genome are limited.<h4>Methods</h4>In this study, the <i>B. orientalis</i> mitochondrial genome was sequenced using both Nanopore PromethION and Illumina NovaSeq 6000 platforms. Genome annotation was performed using MITOFY and MFANNOT, with structural visualization via OGDRAW. In-depth analyses were conducted, including assessments of non-synonymous/synonymous mutation ratios (Ka/Ks), codon usage bias, repeat sequence identification, RNA editing site prediction, collinearity, and the2025-01-01T00:00:00Z20252026-04-08T19:46:05.859Z2025-07-12T03:04:40.337ZS-EPMC119656124018255010.3389/fpls.2025.1534171