biostudies-literatureLi JResearch Project of National Clinical Research Base of Traditional Chinese Medicine of Henan Provincial Health Commission in 2022Henan Province traditional Chinese medicine double first class to create a special project for scientific research in 2023Doctoral Research Start up Fund of the First Affiliated Hospital of Henan University of Traditional Chinese Medicine in 2022332https://www.ebi.ac.uk/biostudies/studies/S-EPMC11969702biostudies-literatureUnknown25(1)<h4>Background</h4>Autism spectrum disorder (ASD) imposes a significant burden on both patients and society. Amino acid metabolism abnormalities are particularly relevant to ASD pathology due to their crucial role in neurotransmitter synthesis, synaptic function, and overall neurodevelopment. This study aims to explore the association between amino acid metabolic abnormalities and the severity of ASD by analyzing the amino acid concentrations in the blood of children with ASD.<h4>Methods</h4>Fasting peripheral blood samples were collected from 344 children with ASD, and amino acid concentrations were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) while strictly following quality control measures. The association between amino acid concentrations and ASD severity wBMC psychiatryAssociations between amino acid levels and autism spectrum disorder severity.PMC119697022022JDZX1132022BSJJ2001HSRP-DFCTCM-2023-5-08Li JMei XFeng GLi PBi LZhang QLi NYang XLiu YYang YWang YZhai PDang WfalseAssociations between amino acid levels and autism spectrum disorder severity.<h4>Background</h4>Autism spectrum disorder (ASD) imposes a significant burden on both patients and society. Amino acid metabolism abnormalities are particularly relevant to ASD pathology due to their crucial role in neurotransmitter synthesis, synaptic function, and overall neurodevelopment. This study aims to explore the association between amino acid metabolic abnormalities and the severity of ASD by analyzing the amino acid concentrations in the blood of children with ASD.<h4>Methods</h4>Fasting peripheral blood samples were collected from 344 children with ASD, and amino acid concentrations were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) while strictly following quality control measures. The association between amino acid concentrations and ASD severity w2025-01-01T00:00:00Z2025 Apr2025-07-08T03:12:45.426Z2025-07-08T03:12:45.426ZS-EPMC119697024018613610.1186/s12888-025-06771-x