{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["Joint Funds for the Innovation of Science and Technology, Fujian Province","Guangdong Basic and Applied Basic Research Foundation Key Project","Science and Technology Special Fund of Guangdong Province of China","the National Natural Science Foundation of China youth science fund projects"],"pagination":["51"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11969778"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(1)"],"pubmed_abstract":["Neoadjuvant checkpoint blockade immunotherapy (NATI) significantly prolonged outcomes for triple-negative breast cancer (TNBC). Residual tumor cells that survive NATI represent high-risk cell populations with metastatic potential and usually evade immunosurveillance by NK cells. Using an 82-protein panel, we here profiled single-cell membrane proteomics of CD56+ (NCAM1+) NK cells from tumor, peri-cancerous tissue, as well as peripheral blood from 28 TNBC patients post-NATI of residual cancer burden II/III. Unsupervised clustering resulted in several distinct clusters: 2 tumor-infiltrating NK (TINK) clusters with divergent functions of immune activation (TNFRSF7+) and suppression (SELL+); 2 immuno-suppressive peri-cancerous clusters; and 1 periphery-specific cluster. Considering the contrad"],"journal":["Breast cancer research : BCR"],"pubmed_title":["Phenotypes and cytokines of NK cells in triple-negative breast cancer resistant to checkpoint blockade immunotherapy."],"pmcid":["PMC11969778"],"funding_grant_id":["2023Y9054","2023B1515230004","STKJ2023002","82102687"],"pubmed_authors":["Ding F","Li J","Lyu X","Ma Y","Chen B","Jiang Y","Zhu P","Huang T","Zheng H","He T","Qi X","Lu J","Niu YS","Zeng J","Zhong G","Wang W","Wang X","Lin J","Wang Y","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypes and cytokines of NK cells in triple-negative breast cancer resistant to checkpoint blockade immunotherapy.","description":"Neoadjuvant checkpoint blockade immunotherapy (NATI) significantly prolonged outcomes for triple-negative breast cancer (TNBC). Residual tumor cells that survive NATI represent high-risk cell populations with metastatic potential and usually evade immunosurveillance by NK cells. Using an 82-protein panel, we here profiled single-cell membrane proteomics of CD56+ (NCAM1+) NK cells from tumor, peri-cancerous tissue, as well as peripheral blood from 28 TNBC patients post-NATI of residual cancer burden II/III. Unsupervised clustering resulted in several distinct clusters: 2 tumor-infiltrating NK (TINK) clusters with divergent functions of immune activation (TNFRSF7+) and suppression (SELL+); 2 immuno-suppressive peri-cancerous clusters; and 1 periphery-specific cluster. Considering the contrad","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-01T03:05:23.164Z","creation":"2025-07-01T03:05:23.164Z"},"accession":"S-EPMC11969778","cross_references":{"pubmed":["40181426"],"doi":["10.1186/s13058-025-02003-y"]}}