biostudies-literatureUnknown15(14)Huynh QT<i>Elatostema tenuicaudatum</i> W. T. Wang, a medicinal plant traditionally utilized in herbal remedies, was explored for its cytotoxic properties. Bioassay-guided fractionation led to the discovery of six novel compounds, designated as elatostemanosides I-VI, with their structures elucidated through advanced spectroscopic methods and DP4+ analysis. Among these, compounds 2, 5, and 6 demonstrated moderate cytotoxicity against the human liver cancer cell line HepG2, exhibiting IC₅₀ values of 18.2 ± 2.1, 32.1 ± 0.4, and 57.6 ± 1.3 µM, respectively. Notably, compound 6 also displayed significant activity against the human breast cancer cell line HCC1806, with an IC₅₀ value of 35.4 ± 0.3 µM. Mechanistic studies revealed these compounds induced apoptosis by modulating the RSC advances10639-10652https://www.ebi.ac.uk/biostudies/studies/S-EPMC11970508biostudies-literatureCytotoxicity-guided isolation of elatostemanosides I-VI from &lt;i&gt;Elatostema tenuicaudatum&lt;/i&gt; W. T. Wang and their cytotoxic activities.PMC11970508Fan YJKuo CCLiu TWPhan TTChu MHNguyen DKHsu SJWang YHLee CKNguyen TVShen YAVo THHuynh QTDuong TTfalseCytotoxicity-guided isolation of elatostemanosides I-VI from &lt;i&gt;Elatostema tenuicaudatum&lt;/i&gt; W. T. Wang and their cytotoxic activities.<i>Elatostema tenuicaudatum</i> W. T. Wang, a medicinal plant traditionally utilized in herbal remedies, was explored for its cytotoxic properties. Bioassay-guided fractionation led to the discovery of six novel compounds, designated as elatostemanosides I-VI, with their structures elucidated through advanced spectroscopic methods and DP4+ analysis. Among these, compounds 2, 5, and 6 demonstrated moderate cytotoxicity against the human liver cancer cell line HepG2, exhibiting IC₅₀ values of 18.2 ± 2.1, 32.1 ± 0.4, and 57.6 ± 1.3 µM, respectively. Notably, compound 6 also displayed significant activity against the human breast cancer cell line HCC1806, with an IC₅₀ value of 35.4 ± 0.3 µM. Mechanistic studies revealed these compounds induced apoptosis by modulating the 2025-01-01T00:00:00Z2025 Apr2025-07-09T03:05:11.283Z2025-07-09T03:05:11.283ZS-EPMC119705084019063210.1039/d4ra09007a