biostudies-literatureUnknown34(9)Shin JAlcohol consumption causes severe liver damage and oxidative stress. This study investigated the hepatoprotective effects of enzymatic porcine placenta hydrolysate (EPPH) in Sprague-Dawley rats under acute alcohol administration. EPPH significantly reduced plasma ethanol and acetaldehyde levels in a dose-dependent manner. Furthermore, EPPH decreased the hepatic levels of malondialdehyde and thiobarbituric acid reactive substances and suppressed <i>Cyp2e1</i> mRNA expression. EPPH decreased the plasma alanine transaminase and aspartate transaminase levels and increased the hepatic NAD⁺/NADH ratio. Hepatic transcriptome analysis revealed the significant regulation of key genes involved in inflammation, alcohol response, and apoptosis. Phosphokinase array analysis demonstrated thatFood science and biotechnology2025-2038https://www.ebi.ac.uk/biostudies/studies/S-EPMC11972273biostudies-literatureActivation of hepatic alcohol metabolism by enzymatic porcine placenta hydrolysate in rats.PMC11972273Ju SHLee SJYoon SMShin JSim DKim YLee YYoo HJung YJKim JSBae GWfalseActivation of hepatic alcohol metabolism by enzymatic porcine placenta hydrolysate in rats.Alcohol consumption causes severe liver damage and oxidative stress. This study investigated the hepatoprotective effects of enzymatic porcine placenta hydrolysate (EPPH) in Sprague-Dawley rats under acute alcohol administration. EPPH significantly reduced plasma ethanol and acetaldehyde levels in a dose-dependent manner. Furthermore, EPPH decreased the hepatic levels of malondialdehyde and thiobarbituric acid reactive substances and suppressed <i>Cyp2e1</i> mRNA expression. EPPH decreased the plasma alanine transaminase and aspartate transaminase levels and increased the hepatic NAD⁺/NADH ratio. Hepatic transcriptome analysis revealed the significant regulation of key genes involved in inflammation, alcohol response, and apoptosis. Phosphokinase array analysis demonstrated that2025-01-01T00:00:00Z2025 May2026-07-16T18:26:57.212Z2026-07-09T11:14:35.826ZS-EPMC119722734019633410.1007/s10068-025-01822-1