{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Baptissart M"],"funding":["Intramural NIH HHS"],"pubmed_abstract":["Changes in the poly(A) tail length of <i>Odf1</i> and other transcripts critical for male fertility have been linked to translational activation during sperm formation <sup>1-3</sup>. The mRNA poly(A) polymerase TENT5C is required for fastening the flagellum to the sperm head, but its role in shaping the poly(A) tail profile of the spermatid transcriptome remains limited <sup>4,5</sup>. Here, we comprehensively document how changes in mRNA poly(A) tail length across the transcriptome reflect transcript metabolism in spermatids. In the absence of TENT5C polymerase activity, the poly(A) tail length of <i>Odf1</i> transcripts is reduced, and the local distribution of ODF1 proteins in spermatids is disrupted. We show that mice expressing a catalytically inactive TENT5C produce headless spermat"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.03.20.644152"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11974682"],"repository":["biostudies-literature"],"pubmed_title":["TENT5C extends &lt;i&gt;Odf1&lt;/i&gt; poly(A) tail to sustain sperm morphogenesis and fertility."],"pmcid":["PMC11974682"],"funding_grant_id":["ZIA ES103339"],"pubmed_authors":["Gupta A","Baptissart M","Poirot AC","Morgan M","Papas BN"],"additional_accession":[]},"is_claimable":false,"name":"TENT5C extends &lt;i&gt;Odf1&lt;/i&gt; poly(A) tail to sustain sperm morphogenesis and fertility.","description":"Changes in the poly(A) tail length of <i>Odf1</i> and other transcripts critical for male fertility have been linked to translational activation during sperm formation <sup>1-3</sup>. The mRNA poly(A) polymerase TENT5C is required for fastening the flagellum to the sperm head, but its role in shaping the poly(A) tail profile of the spermatid transcriptome remains limited <sup>4,5</sup>. Here, we comprehensively document how changes in mRNA poly(A) tail length across the transcriptome reflect transcript metabolism in spermatids. In the absence of TENT5C polymerase activity, the poly(A) tail length of <i>Odf1</i> transcripts is reduced, and the local distribution of ODF1 proteins in spermatids is disrupted. We show that mice expressing a catalytically inactive TENT5C produce headless spermat","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-04-08T19:52:00.701Z","creation":"2026-04-08T14:31:16.757Z"},"accession":"S-EPMC11974682","cross_references":{"pubmed":["40196629"],"doi":["10.1101/2025.03.20.644152"]}}