{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["SoRelle J"],"funding":["NIDDK NIH HHS","NIAID NIH HHS"],"pubmed_abstract":["Intestinal homeostasis relies on a protective mucus layer that separates bacteria from the host, with Muc2 as its primary component. This secreted, gel-forming mucin is heavily O-glycosylated, allowing it to retain water and support beneficial bacteria. For the first time, we demonstrate that Muc2 N-glycosylation plays a critical in mucin maturation, O-glycosylation, barrier integrity, and the prevention of dysbiosis. Using mouse models with global and intestine-specific N-glycan deficiency- caused by the loss of the mannose producing enzyme, Mpi- we uncover an unexpected link between N-glycosylation and intestinal homeostasis. Our findings reveal that Mpi hypomorphic mice are highly sensitive to DSS-induced colitis, while Mpiflox; VillinCre mice spontaneously develop disease, exhibiting i"],"journal":["Research square"],"pagination":["rs.3.rs-6222474"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11975007"],"repository":["biostudies-literature"],"pubmed_title":["N-glycosylation enzyme Mpi is essential for mucin O-glycosylation, host-microbe homeostasis, Paneth cell defense, and metabolism."],"pmcid":["PMC11975007"],"funding_grant_id":["R01 AI125581","U19 AI100627","R01 DK135511","K08 AI155832"],"pubmed_authors":["Argula T","Beutler B","Chen Z","Cho HJ","Roy A","Meregini S","Zaki A","SoRelle J"],"additional_accession":[]},"is_claimable":false,"name":"N-glycosylation enzyme Mpi is essential for mucin O-glycosylation, host-microbe homeostasis, Paneth cell defense, and metabolism.","description":"Intestinal homeostasis relies on a protective mucus layer that separates bacteria from the host, with Muc2 as its primary component. This secreted, gel-forming mucin is heavily O-glycosylated, allowing it to retain water and support beneficial bacteria. For the first time, we demonstrate that Muc2 N-glycosylation plays a critical in mucin maturation, O-glycosylation, barrier integrity, and the prevention of dysbiosis. Using mouse models with global and intestine-specific N-glycan deficiency- caused by the loss of the mannose producing enzyme, Mpi- we uncover an unexpected link between N-glycosylation and intestinal homeostasis. Our findings reveal that Mpi hypomorphic mice are highly sensitive to DSS-induced colitis, while Mpiflox; VillinCre mice spontaneously develop disease, exhibiting i","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-07-03T03:04:43.811Z","creation":"2025-07-03T03:04:43.811Z"},"accession":"S-EPMC11975007","cross_references":{"pubmed":["40195978"],"doi":["10.21203/rs.3.rs-6222474/v1"]}}