biostudies-literatureHuang TJNatural Science Foundation of Jiangxi ProvinceHealth Commission of Jiangxi Province142https://www.ebi.ac.uk/biostudies/studies/S-EPMC11977089biostudies-literatureUnknown82(1)<h4>Background</h4>Sarcopenia, the age-related decline in muscle mass and function, poses a major health risk to the elderly population. Although dietary advanced glycation end-products (AGEs) have been implicated in worsening sarcopenia, the precise molecular mechanisms remain unclear.<h4>Methods</h4>A sarcopenia animal model was established by feeding a high AGE diet to C57BL/6 mice. Muscle function and mass were assessed using grip strength tests, and rotarod tests. Proteomic analysis was used to identify differentially expressed proteins. Immunoprecipitation, mass spectrometry, and co-immunoprecipitation were employed to investigate protein interactions both in vivo and in vitro. Quantitative reverse transcription PCR and Western blotting were conducted to measure gene and protein exprCellular and molecular life sciences : CMLSDietary advanced glycation end-products exacerbate sarcopenia onset by activating apoptosis through PRMT1-mediated CRTC3 arginine methylation.PMC1197708920242BAB25539202310009Wan QHuang TJLi QLi YJZheng JNShang SChen FXfalseDietary advanced glycation end-products exacerbate sarcopenia onset by activating apoptosis through PRMT1-mediated CRTC3 arginine methylation.<h4>Background</h4>Sarcopenia, the age-related decline in muscle mass and function, poses a major health risk to the elderly population. Although dietary advanced glycation end-products (AGEs) have been implicated in worsening sarcopenia, the precise molecular mechanisms remain unclear.<h4>Methods</h4>A sarcopenia animal model was established by feeding a high AGE diet to C57BL/6 mice. Muscle function and mass were assessed using grip strength tests, and rotarod tests. Proteomic analysis was used to identify differentially expressed proteins. Immunoprecipitation, mass spectrometry, and co-immunoprecipitation were employed to investigate protein interactions both in vivo and in vitro. Quantitative reverse transcription PCR and Western blotting were conducted to measure gene and protein expr2025-01-01T00:00:00Z2025 Apr2025-07-13T03:05:11.231Z2025-07-13T03:05:11.231ZS-EPMC119770894019280110.1007/s00018-025-05657-1