biostudies-literatureUnknown13Ivanovska A<h4>Objective</h4>Osteoarthritis (OA) is a widespread and debilitating joint disease characterized by synovial inflammation, cartilage degeneration, and chronic joint pain. Mesenchymal stromal cells (MSCs) have shown therapeutic efficacy for many diseases with a strong inflammatory profile, including OA. However, the disease-specific mechanisms of action underpinning the effects of post-local MSC delivery remain unaddressed. In this study, we aimed to characterize the disease-induced profile of MSCs following exposure to the <i>in vivo</i> osteoarthritis environment.<h4>Methods</h4>Murine syngeneic GFP + bone marrow-derived MSCs (BM-MSCs) were delivered <i>via</i> intra-articular injection in a mouse collagenase-induced osteoarthritis (CIOA) model (n = 8). BM-MSCs were retrieved by cell soFrontiers in cell and developmental biology1521437https://www.ebi.ac.uk/biostudies/studies/S-EPMC11980424biostudies-literatureIdentification of disease-stage therapeutic responses of mesenchymal stromal cells retrieved from murine osteoarthritic joints.PMC11980424Burke AMorris DWEnglish KMcLoughlin SMukeria EReilly JRitter TRaman SO'Brien AJohnstone BHennessy CDooley CRyan AEKamath RMancuso PMurphy JMShaw GRiet DVBarry FIvanovska ALevesque MCHawthorne IfalseIdentification of disease-stage therapeutic responses of mesenchymal stromal cells retrieved from murine osteoarthritic joints.<h4>Objective</h4>Osteoarthritis (OA) is a widespread and debilitating joint disease characterized by synovial inflammation, cartilage degeneration, and chronic joint pain. Mesenchymal stromal cells (MSCs) have shown therapeutic efficacy for many diseases with a strong inflammatory profile, including OA. However, the disease-specific mechanisms of action underpinning the effects of post-local MSC delivery remain unaddressed. In this study, we aimed to characterize the disease-induced profile of MSCs following exposure to the <i>in vivo</i> osteoarthritis environment.<h4>Methods</h4>Murine syngeneic GFP + bone marrow-derived MSCs (BM-MSCs) were delivered <i>via</i> intra-articular injection in a mouse collagenase-induced osteoarthritis (CIOA) model (n = 8). BM-MSCs were retrieved by cell so2025-01-01T00:00:00Z20252025-07-07T03:09:01.894Z2025-07-07T03:09:01.894ZS-EPMC119804244020640310.3389/fcell.2025.1521437