biostudies-literatureSong HKey Research and Development Program of Heilongjiang ProvinceNn10 project at Harbin Medical University Cancer HospitalSpring Swallow Support Program for Young Talents from Heilongjiang Provincial Department of Science and TechnologyHeilongjiang Provincial Department of Human Resources and Social SecurityNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationHarbin Medical University Cancer Hospital Haiyan FoundationNational Key Research and Development Program of China500https://www.ebi.ac.uk/biostudies/studies/S-EPMC11982012biostudies-literatureUnknown16(1)<h4>Purpose</h4>Bladder cancer (BC) is characterized by high heterogeneity, with non-muscle-invasive (NMIBC) and muscle-invasive (MIBC) stages differing significantly in clinical behavior and outcomes. The transition from NMIBC to MIBC involves extensive tumor microenvironment (TME) remodeling, particularly in endothelial cells (ECs), which drive angiogenesis and modulate immune and extracellular matrix (ECM) interactions. However, the precise roles of ECs in this progression remain poorly defined.<h4>Methods</h4>Public single-cell RNA sequencing (scRNA-seq) datasets from 47 BC patients were analyzed to characterize endothelial cell heterogeneity and functional states across NMIBC and MIBC. Computational tools such as CellChat were applied to reconstruct cell-cell communication networks, fDiscover oncologyA single-cell atlas of bladder cancer unveils dynamic cellular composition and endothelial functional shifts during progression.PMC119820122022ZX103C072022ZX06C04LBH-Z220302021M693828Nn102024-01ZD2024-24821719992021YFB3801000CYQN24038Li HHu XShi QTong ZSong HZhao YYin ZXie GLi YYang ZLiu ZWang ZXu WfalseA single-cell atlas of bladder cancer unveils dynamic cellular composition and endothelial functional shifts during progression.<h4>Purpose</h4>Bladder cancer (BC) is characterized by high heterogeneity, with non-muscle-invasive (NMIBC) and muscle-invasive (MIBC) stages differing significantly in clinical behavior and outcomes. The transition from NMIBC to MIBC involves extensive tumor microenvironment (TME) remodeling, particularly in endothelial cells (ECs), which drive angiogenesis and modulate immune and extracellular matrix (ECM) interactions. However, the precise roles of ECs in this progression remain poorly defined.<h4>Methods</h4>Public single-cell RNA sequencing (scRNA-seq) datasets from 47 BC patients were analyzed to characterize endothelial cell heterogeneity and functional states across NMIBC and MIBC. Computational tools such as CellChat were applied to reconstruct cell-cell communication networks, f2025-01-01T00:00:00Z2025 Apr2026-07-15T08:48:38.171Z2026-07-02T03:08:56.462ZS-EPMC119820124020527410.1007/s12672-025-02297-6