biostudies-literatureUnknown2025Liu MC<b>Background:</b> Reduction of <i>Faecalibacterium prausnitzii</i> abundance is related to inflammatory bowel diseases (IBDs), and supplement of it exists protective effects. <b>Aim:</b> This study aimed to establish a <i>F. prausnitzii</i>-colonized mouse model and investigate that the presence of <i>F. prausnitzii</i> in the gut can ameliorate the severity of dextran sulfate sodium (DSS)-induced colitis. <b>Methods:</b> A <i>F. prausnitzii</i> (ATCC 27768) strain was maintained on the PS-BHI agar plates and manipulated in a strictly anaerobic chamber. A <i>F. prausnitzii</i>-colonized C57BL/6 mice model was tested by a rectal enema with 1 × 10<sup>9</sup> bacteria/day for 3 days. The 5% DSS was added to drinking water for 3 days to induce colitis and diarrhea in experimental mice. The cMediators of inflammation7280675https://www.ebi.ac.uk/biostudies/studies/S-EPMC11986197biostudies-literature<i>Faecalibacterium prausnitzii</i> Colonization Attenuates Gut Inflammation and Epithelial Damage in a DSS-Induced Colitis Mice Model.PMC11986197Liu MCTseng HYYu YTWang YCShu YATsai PJYang YJLi MJCheng HCfalse<i>Faecalibacterium prausnitzii</i> Colonization Attenuates Gut Inflammation and Epithelial Damage in a DSS-Induced Colitis Mice Model.<b>Background:</b> Reduction of <i>Faecalibacterium prausnitzii</i> abundance is related to inflammatory bowel diseases (IBDs), and supplement of it exists protective effects. <b>Aim:</b> This study aimed to establish a <i>F. prausnitzii</i>-colonized mouse model and investigate that the presence of <i>F. prausnitzii</i> in the gut can ameliorate the severity of dextran sulfate sodium (DSS)-induced colitis. <b>Methods:</b> A <i>F. prausnitzii</i> (ATCC 27768) strain was maintained on the PS-BHI agar plates and manipulated in a strictly anaerobic chamber. A <i>F. prausnitzii</i>-colonized C57BL/6 mice model was tested by a rectal enema with 1 × 10<sup>9</sup> bacteria/day for 3 days. The 5% DSS was added to drinking water for 3 days to induce colitis and diarrhea in experimental mice. The c2025-01-01T00:00:00Z20252025-07-08T03:10:16.362Z2025-07-08T03:10:16.362ZS-EPMC119861974022448410.1155/mi/7280675