biostudies-literatureUnknown57(3)Wang JThe persistent global burden of hepatitis B virus (HBV) infection has prompted ongoing investigations into host determinants of viral control. In this study, we investigate the regulatory influence of the host gene cleavage stimulation factor subunit 2 (CSTF2) on HBV replication dynamics. We demonstrate differential CSTF2 expression across the spectrum of HBV infection phases, with upregulated expression noted during the immune-reactive and inactive carrier states compared with the immune-tolerant phase. Notably, dose-responsive attenuation of HBV DNA, as well as surface and core protein levels, is observed subsequent to CSTF2 overexpression, whereas HBV RNA levels remain unaffected. Upon HBV transfection, a notable alteration in CSTF2 subcellular localization is discerned, suggesting actiActa biochimica et biophysica Sinica486-496https://www.ebi.ac.uk/biostudies/studies/S-EPMC11986456biostudies-literatureThe host gene <i>CSTF2</i> regulates HBV replication via HBV PRE-induced nuclear export.PMC11986456Wu JXie WLu MLi JShen ZZhang JMao RWang JfalseThe host gene <i>CSTF2</i> regulates HBV replication via HBV PRE-induced nuclear export.The persistent global burden of hepatitis B virus (HBV) infection has prompted ongoing investigations into host determinants of viral control. In this study, we investigate the regulatory influence of the host gene cleavage stimulation factor subunit 2 (CSTF2) on HBV replication dynamics. We demonstrate differential CSTF2 expression across the spectrum of HBV infection phases, with upregulated expression noted during the immune-reactive and inactive carrier states compared with the immune-tolerant phase. Notably, dose-responsive attenuation of HBV DNA, as well as surface and core protein levels, is observed subsequent to CSTF2 overexpression, whereas HBV RNA levels remain unaffected. Upon HBV transfection, a notable alteration in CSTF2 subcellular localization is discerned, suggesting acti2024-01-01T00:00:00Z2024 Dec2026-06-01T20:14:31.035Z2025-07-03T03:04:41.656ZS-EPMC119864563972257210.3724/abbs.2024216