biostudies-literatureMahayri TMSardegna Foundation105037https://www.ebi.ac.uk/biostudies/studies/S-EPMC11987624biostudies-literatureUnknown104(5)The effect of the dietary inclusion of Hermetia illucens larvae meal on the diversity of the methanogenic archaea in the caecum of laying hens (Hy-line Brown) was investigated using molecular methods. A total of 27 hens, selected equally for slaughter from 162 birds which were divided equally into 3 treatment groups including control group C with a diet containing corn-soybean meal and 2 experimental groups, HI25 and HI50, in which 25% and 50% of the soybean meal protein was replaced by the protein from a Hermetia illucens larvae meal, respectively. At 40 weeks of age, the methanogenic community of caecal content of 9 hens per group was analyzed using a 16S rRNA gene clone library. A total of 108 positive clones, 35 from the control group, 44 from the HI25 group and 29 from the HI50 group,Poultry scienceThe inclusion of insect meal from Hermetia illucens larvae in the diet of laying hens (Hy-line Brown) affects the caecal diversity of methanogenic archaea.PMC11987624FDS2223MONIELLO \u2013 CUP J83C22000160007Bovera FMrazek JPiccolo GMurgia GAMahayri TMFliegerova KOMoniello GfalseThe inclusion of insect meal from Hermetia illucens larvae in the diet of laying hens (Hy-line Brown) affects the caecal diversity of methanogenic archaea.The effect of the dietary inclusion of Hermetia illucens larvae meal on the diversity of the methanogenic archaea in the caecum of laying hens (Hy-line Brown) was investigated using molecular methods. A total of 27 hens, selected equally for slaughter from 162 birds which were divided equally into 3 treatment groups including control group C with a diet containing corn-soybean meal and 2 experimental groups, HI25 and HI50, in which 25% and 50% of the soybean meal protein was replaced by the protein from a Hermetia illucens larvae meal, respectively. At 40 weeks of age, the methanogenic community of caecal content of 9 hens per group was analyzed using a 16S rRNA gene clone library. A total of 108 positive clones, 35 from the control group, 44 from the HI25 group and 29 from the HI50 group,2025-01-01T00:00:00Z2025 May2026-05-26T20:06:46.389Z2025-07-06T03:04:31.082ZS-EPMC119876244012025010.1016/j.psj.2025.105037