biostudies-literatureWu YFujian Provincial Natural Science FoundationNational Natural Science Foundation of ChinaInitial Research Fund of Jimei University1260https://www.ebi.ac.uk/biostudies/studies/S-EPMC11988977biostudies-literatureUnknown14(7)Gray mold, caused by <i>Botrytis cinerea</i>, is the primary factor contributing to postharvest losses in table grape fruit. In this study, we have identified amphotericin B (AMB), a natural compound originating from <i>Streptomyces nodosus</i>, as a promising agent in managing postharvest gray mold in table grapes. In vitro experiments demonstrated that 0.2 mg/L AMB achieved an inhibition rate of over 90% against <i>B. cinerea</i> in PDA medium, and in vivo assays on grape berries showed that 200 mg/L AMB treatment could completely suppress the occurrence of gray mold disease. A mechanism analysis found that AMB treatment disrupted the plasma membrane structure, which consequently triggered cellular leakage and induced cell death. Furthermore, AMB application effectively modulated the traFoods (Basel, Switzerland)Mechanism Analysis of Amphotericin B Controlling Postharvest Gray Mold in Table Grapes.PMC11988977ZQ2022011322025652022J05151Ke YWang SRen TWang YWang JWu YfalseMechanism Analysis of Amphotericin B Controlling Postharvest Gray Mold in Table Grapes.Gray mold, caused by <i>Botrytis cinerea</i>, is the primary factor contributing to postharvest losses in table grape fruit. In this study, we have identified amphotericin B (AMB), a natural compound originating from <i>Streptomyces nodosus</i>, as a promising agent in managing postharvest gray mold in table grapes. In vitro experiments demonstrated that 0.2 mg/L AMB achieved an inhibition rate of over 90% against <i>B. cinerea</i> in PDA medium, and in vivo assays on grape berries showed that 200 mg/L AMB treatment could completely suppress the occurrence of gray mold disease. A mechanism analysis found that AMB treatment disrupted the plasma membrane structure, which consequently triggered cellular leakage and induced cell death. Furthermore, AMB application effectively modulated the tra2025-01-01T00:00:00Z2025 Apr2025-07-10T03:08:11.975Z2025-07-10T03:08:11.975ZS-EPMC119889774023854310.3390/foods14071260