biostudies-literatureHuang Jthe Seed Industry Innovation and Industrialization in Fujian Provincethe Minjiang University Introduces Talent pre-research projectthe Science and Technology Project of Fuzhou institute of Oceanography3235https://www.ebi.ac.uk/biostudies/studies/S-EPMC11989308biostudies-literatureUnknown26(7)Reproduction and breeding are crucial to maintaining abalone aquaculture. Understanding the molecular underpinnings of sexual maturation is essential for advancing knowledge in reproductive biology. However, the molecular mechanisms of gonadal development in abalones remain poorly understood, particularly in microRNA (miRNA)-mediated regulation. Thus, this study conducted a comprehensive transcriptomic analysis of abalone <i>Haliotis discus hannai</i> (<i>H. discus hannai</i>) to identify genes and miRNAs associated with ovarian and testicular discovery. This study identified 685 differentially expressed (DE) genes between the <i>H. discus hannai</i> ovary (DD_ovary) and testis (DD_testis) groups, comprising 479 upregulated and 206 downregulated genes in the DD_ovary. Moreover, 137 miRNAs,International journal of molecular sciencesIntegrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (<i>Haliotis discus hannai</i>).PMC11989308No.2025F15No.MJY22008, No.MJY24020No.2021FJSCZY02Zhou MHuang JChen JShe ZKe CfalseIntegrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (<i>Haliotis discus hannai</i>).Reproduction and breeding are crucial to maintaining abalone aquaculture. Understanding the molecular underpinnings of sexual maturation is essential for advancing knowledge in reproductive biology. However, the molecular mechanisms of gonadal development in abalones remain poorly understood, particularly in microRNA (miRNA)-mediated regulation. Thus, this study conducted a comprehensive transcriptomic analysis of abalone <i>Haliotis discus hannai</i> (<i>H. discus hannai</i>) to identify genes and miRNAs associated with ovarian and testicular discovery. This study identified 685 differentially expressed (DE) genes between the <i>H. discus hannai</i> ovary (DD_ovary) and testis (DD_testis) groups, comprising 479 upregulated and 206 downregulated genes in the DD_ovary. Moreover, 137 miRNAs,2025-01-01T00:00:00Z2025 Mar2026-04-08T19:42:51.985Z2025-07-14T03:02:43.481ZS-EPMC119893084024407010.3390/ijms26073235