{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhuang J"],"funding":["Hubei Provincial Department of Science and Technology"],"pagination":["3215"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11989904"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(7)"],"pubmed_abstract":["This study investigates the biochemical properties of two xylanases, ZgXyn10A and CaXyn10B, which are members of the glycoside hydrolase family 10 (GH10) and originate from the marine Bacteroidetes species <i>Zobellia galactanivorans</i> and <i>Cellulophaga algicola</i>, respectively. Utilizing an auto-induction expression system in <i>Escherichia coli</i>, high-purity recombinant forms of these enzymes were successfully produced. Biochemical assays revealed that ZgXyn10A and CaXyn10B exhibit optimal activities at 40 °C and 30 °C, respectively, and demonstrate a high sensitivity to temperature fluctuations. Unlike conventional low-temperature enzymes, these xylanases retain only a fraction of their maximal activity at lower temperatures. To gain deeper insights into the structural and func"],"journal":["International journal of molecular sciences"],"pubmed_title":["Loop Dynamics Mediate Thermal Adaptation of Two Xylanases from Marine Bacteria."],"pmcid":["PMC11989904"],"funding_grant_id":["2024JDC050"],"pubmed_authors":["Yang J","Zhang Y","Han Z","Wang Y","Zhuang J"],"additional_accession":[]},"is_claimable":false,"name":"Loop Dynamics Mediate Thermal Adaptation of Two Xylanases from Marine Bacteria.","description":"This study investigates the biochemical properties of two xylanases, ZgXyn10A and CaXyn10B, which are members of the glycoside hydrolase family 10 (GH10) and originate from the marine Bacteroidetes species <i>Zobellia galactanivorans</i> and <i>Cellulophaga algicola</i>, respectively. Utilizing an auto-induction expression system in <i>Escherichia coli</i>, high-purity recombinant forms of these enzymes were successfully produced. Biochemical assays revealed that ZgXyn10A and CaXyn10B exhibit optimal activities at 40 °C and 30 °C, respectively, and demonstrate a high sensitivity to temperature fluctuations. Unlike conventional low-temperature enzymes, these xylanases retain only a fraction of their maximal activity at lower temperatures. To gain deeper insights into the structural and func","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-04-08T19:01:12.332Z","creation":"2025-07-02T03:05:03.122Z"},"accession":"S-EPMC11989904","cross_references":{"pubmed":["40244048"],"doi":["10.3390/ijms26073215"]}}