biostudies-literatureZhuang JHubei Provincial Department of Science and Technology3215https://www.ebi.ac.uk/biostudies/studies/S-EPMC11989904biostudies-literatureUnknown26(7)This study investigates the biochemical properties of two xylanases, ZgXyn10A and CaXyn10B, which are members of the glycoside hydrolase family 10 (GH10) and originate from the marine Bacteroidetes species <i>Zobellia galactanivorans</i> and <i>Cellulophaga algicola</i>, respectively. Utilizing an auto-induction expression system in <i>Escherichia coli</i>, high-purity recombinant forms of these enzymes were successfully produced. Biochemical assays revealed that ZgXyn10A and CaXyn10B exhibit optimal activities at 40 °C and 30 °C, respectively, and demonstrate a high sensitivity to temperature fluctuations. Unlike conventional low-temperature enzymes, these xylanases retain only a fraction of their maximal activity at lower temperatures. To gain deeper insights into the structural and funcInternational journal of molecular sciencesLoop Dynamics Mediate Thermal Adaptation of Two Xylanases from Marine Bacteria.PMC119899042024JDC050Yang JZhang YHan ZWang YZhuang JfalseLoop Dynamics Mediate Thermal Adaptation of Two Xylanases from Marine Bacteria.This study investigates the biochemical properties of two xylanases, ZgXyn10A and CaXyn10B, which are members of the glycoside hydrolase family 10 (GH10) and originate from the marine Bacteroidetes species <i>Zobellia galactanivorans</i> and <i>Cellulophaga algicola</i>, respectively. Utilizing an auto-induction expression system in <i>Escherichia coli</i>, high-purity recombinant forms of these enzymes were successfully produced. Biochemical assays revealed that ZgXyn10A and CaXyn10B exhibit optimal activities at 40 °C and 30 °C, respectively, and demonstrate a high sensitivity to temperature fluctuations. Unlike conventional low-temperature enzymes, these xylanases retain only a fraction of their maximal activity at lower temperatures. To gain deeper insights into the structural and func2025-01-01T00:00:00Z2025 Mar2026-04-08T19:01:12.332Z2025-07-02T03:05:03.122ZS-EPMC119899044024404810.3390/ijms26073215