biostudies-literatureSong Pthe National Key R&D Program of Chinathe National Science and Technology innovation 20303339https://www.ebi.ac.uk/biostudies/studies/S-EPMC11989977biostudies-literatureUnknown26(7)<i>Fusarium</i> head blight (FHB), caused by <i>Fusarium graminearum</i>, is a globally significant disease that severely impacts the yield and quality of wheat. Breeding resistant wheat varieties using resistance genes is the most cost-effective strategy for managing FHB, but few markers are available for marker-assisted selection (MAS) of resistance. In this study, we evaluated the resistance of a recombinant inbred line (RIL) population to FHB through single-floret inoculation in four field environments over two years. Combined with quantitative trait loci (QTL) detection through high-density genetic mapping based on wheat 50 K SNP arrays, we identified a total of 21 QTLs influencing FHB resistance. It is worth noting that <i>QFhba-5D.2-1</i> was detected in two field environments as weInternational journal of molecular sciencesExploration of Genomic Regions Associated with Fusarium Head Blight Resistance in Wheat and Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers.PMC119899772016YFD0101802No.2023ZD040230306Li HXing YGuo XFeng YZhao WSong KLi YSong PZhang XSun SSun DWang XWang ZZhang AZhao HfalseExploration of Genomic Regions Associated with Fusarium Head Blight Resistance in Wheat and Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers.<i>Fusarium</i> head blight (FHB), caused by <i>Fusarium graminearum</i>, is a globally significant disease that severely impacts the yield and quality of wheat. Breeding resistant wheat varieties using resistance genes is the most cost-effective strategy for managing FHB, but few markers are available for marker-assisted selection (MAS) of resistance. In this study, we evaluated the resistance of a recombinant inbred line (RIL) population to FHB through single-floret inoculation in four field environments over two years. Combined with quantitative trait loci (QTL) detection through high-density genetic mapping based on wheat 50 K SNP arrays, we identified a total of 21 QTLs influencing FHB resistance. It is worth noting that <i>QFhba-5D.2-1</i> was detected in two field environments as we2025-01-01T00:00:00Z2025 Apr2025-07-10T03:08:07.522Z2025-07-10T03:08:07.522ZS-EPMC119899774024422510.3390/ijms26073339