biostudies-literatureUnknown30(7)Otsuki KGelatin from deer has garnered attention as a high-value health-promoting resource given its history of usage as a traditional Chinese medicine and recent studies demonstrating its biological activities. Mass spectrometry-based methods have increasingly been employed for species identification in collagen-based materials, effectively addressing challenges in quality control and authenticity verification. This study aims to identify characteristic marker peptides in gelatins from sika deer (<i>Cervus nippon</i>) to support their effective use as a health-promoting resource. Gelatin samples were enzymatically digested, and the resulting peptide mixtures were analyzed using ultra-high-performance liquid chromatography coupled with quadrupole Q-Exactive-Orbitrap mass spectrometry (UHPLC-Q-ExacMolecules (Basel, Switzerland)1528https://www.ebi.ac.uk/biostudies/studies/S-EPMC11990231biostudies-literatureIdentification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography-Quadrupole-Exactive-Orbitrap Mass Spectrometry.PMC11990231Li WNomizo ALi DZhang MKikuchi TOtsuki KfalseIdentification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography-Quadrupole-Exactive-Orbitrap Mass Spectrometry.Gelatin from deer has garnered attention as a high-value health-promoting resource given its history of usage as a traditional Chinese medicine and recent studies demonstrating its biological activities. Mass spectrometry-based methods have increasingly been employed for species identification in collagen-based materials, effectively addressing challenges in quality control and authenticity verification. This study aims to identify characteristic marker peptides in gelatins from sika deer (<i>Cervus nippon</i>) to support their effective use as a health-promoting resource. Gelatin samples were enzymatically digested, and the resulting peptide mixtures were analyzed using ultra-high-performance liquid chromatography coupled with quadrupole Q-Exactive-Orbitrap mass spectrometry (UHPLC-Q-Exac2025-01-01T00:00:00Z2025 Mar2026-04-14T03:26:32.243Z2025-07-03T03:04:20.979ZS-EPMC119902314028613110.3390/molecules30071528