{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo D"],"funding":["\"Xingdian Talent Support Program\" of Yunnan Province","Yunnan Fundamental Research Projects","Natural Science Foundation of Yunnan Province","National Natural Science Foundation of China","“Xingdian Talent Support Program” of Yunnan Province"],"pagination":["gkaf302"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11995263"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(7)"],"pubmed_abstract":["Two-cell-like cells (2CLCs), a rare population (∼0.5%) in mouse embryonic stem cell (mESC) cultures, are in a transient totipotent-like state resembling that of 2C-stage embryos, and their discovery and characterization have greatly facilitated the study of early developmental events, such as zygotic genome activation. However, the molecular determinants governing 2C-like reprogramming remain to be elucidated. Here, we show that ZBTB24, CDCA7, and HELLS, components of a molecular pathway that is involved in the pathogenesis of immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, function as negative regulators of 2C-like reprogramming by maintaining DNA methylation of the Dux cluster, a master inducer of the 2C-like state. Disruption of the ZBTB24-CDCA7-HELLS axi"],"journal":["Nucleic acids research"],"pubmed_title":["The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression."],"pmcid":["PMC11995263"],"funding_grant_id":["202301AT070304","202201BE070001-006","KKRD202273103","202102AA100053","32270845"],"pubmed_authors":["Dan J","Liu Y","Zhang J","Lu Y","Hardikar S","Guo D","Du Z","Lin M","Xue Y","Chen T"],"additional_accession":[]},"is_claimable":false,"name":"The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression.","description":"Two-cell-like cells (2CLCs), a rare population (∼0.5%) in mouse embryonic stem cell (mESC) cultures, are in a transient totipotent-like state resembling that of 2C-stage embryos, and their discovery and characterization have greatly facilitated the study of early developmental events, such as zygotic genome activation. However, the molecular determinants governing 2C-like reprogramming remain to be elucidated. Here, we show that ZBTB24, CDCA7, and HELLS, components of a molecular pathway that is involved in the pathogenesis of immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, function as negative regulators of 2C-like reprogramming by maintaining DNA methylation of the Dux cluster, a master inducer of the 2C-like state. Disruption of the ZBTB24-CDCA7-HELLS axi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-15T08:56:09.919Z","creation":"2026-07-02T03:08:38.854Z"},"accession":"S-EPMC11995263","cross_references":{"pubmed":["40226918"],"doi":["10.1093/nar/gkaf302"]}}