{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Wei Y"],"pubmed_abstract":["<h4>Introduction</h4>Phospholipase C (PLC) is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phospholipase-specific PLC (PI-PLC) and non-specific PLC (NPC) depending on its specific substrate.<h4>Methods</h4>In this study, neomycin sulfate (NS, 100 mM) was used to inhibit the activity of phospholipase C in maize seedlings, and the effect of phospholipase C on lipid metabolism was investigated by combined analysis of transcriptome and lipidome.<h4>Results</h4>Lipidomic analysis showed that when PLC was inhibited, the content of phospholipids showed more than 10% increase due to the elevated accumulation of PC and PE. At the same time, transcriptomic data suggested an activation of the de novo biosynthesis of PC and PE from choline and ethanolamine v"],"journal":["Frontiers in plant science"],"pagination":["1547477"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11996868"],"repository":["biostudies-literature"],"pubmed_title":["Effects of phospholipase C inhibition on the regulation of membrane lipid metabolism in maize leaves."],"pmcid":["PMC11996868"],"pubmed_authors":["Ge S","Guo X","Zhang H","Cai Z","Che X","Deng J","Che Z","He L","Wei Y","Xu J"],"additional_accession":[]},"is_claimable":false,"name":"Effects of phospholipase C inhibition on the regulation of membrane lipid metabolism in maize leaves.","description":"<h4>Introduction</h4>Phospholipase C (PLC) is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phospholipase-specific PLC (PI-PLC) and non-specific PLC (NPC) depending on its specific substrate.<h4>Methods</h4>In this study, neomycin sulfate (NS, 100 mM) was used to inhibit the activity of phospholipase C in maize seedlings, and the effect of phospholipase C on lipid metabolism was investigated by combined analysis of transcriptome and lipidome.<h4>Results</h4>Lipidomic analysis showed that when PLC was inhibited, the content of phospholipids showed more than 10% increase due to the elevated accumulation of PC and PE. At the same time, transcriptomic data suggested an activation of the de novo biosynthesis of PC and PE from choline and ethanolamine v","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2025-07-14T03:02:53.008Z","creation":"2025-07-14T03:02:53.008Z"},"accession":"S-EPMC11996868","cross_references":{"pubmed":["40235914"],"doi":["10.3389/fpls.2025.1547477"]}}