biostudies-literatureLin YZNational Science and Technology Council, TaiwanMinistry of Education (MOE), TaiwanChina Medical University HospitalChina Medical Universitye010363https://www.ebi.ac.uk/biostudies/studies/S-EPMC11997822biostudies-literatureUnknown13(4)<h4>Background</h4>MicroRNAs (miRNAs) are epigenetic regulators of T cell maturation and exhaustion. However, the mechanisms by which miRNAs influence T cell function in tumor environments remain unclear. This study focuses on miR-379-5p, which counteracts T cell exhaustion and enhances antitumor responses.<h4>Methods</h4>Native CD8⁺ T cells were isolated from the blood of healthy donors and subjected to chronic stimulation to induce exhaustion. RNA sequencing and miRNA sequencing were performed to identify differentially expressed miRNAs. These miRNAs underwent bioinformatics analyses, including DESeq enrichment, immune cell infiltration assessment, and patient prognostic outcomes in The Cancer Genome Atlas data sets to assess their potential involvement in T cell exhaustion anJournal for immunotherapy of cancerMemory-promoting function of miR-379-5p attenuates CD8&lt;sup&gt;+&lt;/sup&gt; T cell exhaustion by targeting immune checkpoints.PMC11997822DMR-113-191, DMR-113-208CMU111-IP-04DMR-113-190NSTC 112-2311-B-039-001NSTC-111-2314-B-039-055-MY3DMR-112-056NSTC 112-2622-E-039-001Higher Education Sprout ProjectCMU112-MF-25Liu CHWu WRLiu LCShen YCZhuo GYLee CCLin YZLiao TYWang SCLi HWCheng WCChung FCfalseMemory-promoting function of miR-379-5p attenuates CD8&lt;sup&gt;+&lt;/sup&gt; T cell exhaustion by targeting immune checkpoints.<h4>Background</h4>MicroRNAs (miRNAs) are epigenetic regulators of T cell maturation and exhaustion. However, the mechanisms by which miRNAs influence T cell function in tumor environments remain unclear. This study focuses on miR-379-5p, which counteracts T cell exhaustion and enhances antitumor responses.<h4>Methods</h4>Native CD8⁺ T cells were isolated from the blood of healthy donors and subjected to chronic stimulation to induce exhaustion. RNA sequencing and miRNA sequencing were performed to identify differentially expressed miRNAs. These miRNAs underwent bioinformatics analyses, including DESeq enrichment, immune cell infiltration assessment, and patient prognostic outcomes in The Cancer Genome Atlas data sets to assess their potential involvement in T cell exhaustion an2025-01-01T00:00:00Z2025 Apr2026-06-02T20:14:20.689Z2025-07-10T03:08:32.934ZS-EPMC119978224022115110.1136/jitc-2024-010363