biostudies-literatureLu MGuangdong Province Medical Science and Technology Research Foundation Project373https://www.ebi.ac.uk/biostudies/studies/S-EPMC11998445biostudies-literatureUnknown20(1)<h4>Background</h4>Osteoarthritis (OA), a degenerative joint disease characterized by cartilage degradation and inflammation, lacks effective disease-modifying therapies. Quercetin, a bioactive flavonoid derived from Traditional Chinese Medicine, exhibits anti-inflammatory and chondroprotective properties but is limited by poor bioavailability. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer a promising strategy for targeted drug delivery and cartilage regeneration.<h4>Methods</h4>Bone marrow-derived MSC exosomes (Que-Exo) were isolated after preconditioning with quercetin (1µM, 24 h). Their effects were evaluated in IL-1β-stimulated chondrocytes via RT-qPCR, Western blot, transcriptomics, and proteomics. An ACLT-induced OA mouse model received intra-articular injections of Que-ExoJournal of orthopaedic surgery and researchQuercetin-primed MSC exosomes synergistically attenuate osteoarthritis progression.PMC11998445A2024661Lou ALu MFan WZhao LLi SGao JHe LfalseQuercetin-primed MSC exosomes synergistically attenuate osteoarthritis progression.<h4>Background</h4>Osteoarthritis (OA), a degenerative joint disease characterized by cartilage degradation and inflammation, lacks effective disease-modifying therapies. Quercetin, a bioactive flavonoid derived from Traditional Chinese Medicine, exhibits anti-inflammatory and chondroprotective properties but is limited by poor bioavailability. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer a promising strategy for targeted drug delivery and cartilage regeneration.<h4>Methods</h4>Bone marrow-derived MSC exosomes (Que-Exo) were isolated after preconditioning with quercetin (1µM, 24 h). Their effects were evaluated in IL-1β-stimulated chondrocytes via RT-qPCR, Western blot, transcriptomics, and proteomics. An ACLT-induced OA mouse model received intra-articular injections of Que-Exo2025-01-01T00:00:00Z2025 Apr2025-07-10T03:08:20.734Z2025-07-10T03:08:20.734ZS-EPMC119984454022979110.1186/s13018-025-05785-1