{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Desligniere E"],"funding":["Dutch Research Council (NWO)"],"pagination":["101454"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11999443"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(2)"],"pubmed_abstract":["The impressive success of mRNA-based vaccines to combat COVID-19 has encouraged biopharmaceutical companies to invest in broader applications of alike vaccines for various diseases. Analytical approaches must keep pace to support this surge in the development of mRNA-based therapies. Intact mass analysis of mid- to large mRNA molecules (>1,000 nt) poses significant analytical challenges due to mRNA size, heterogeneity, and instability. Here, we demonstrate how single-particle Orbitrap-based charge detection mass spectrometry (CDMS) and mass photometry (MP) approaches can rapidly measure the mass of various intact high-mass capped mRNAs, up to 9,400 nt (∼3 MDa) in size. While ensemble MS yielded approximate masses for mRNAs <2,000 nt, it failed to provide information on samples of longer se"],"journal":["Molecular therapy. Methods & clinical development"],"pubmed_title":["Characterization of intact mRNA-based therapeutics by charge detection mass spectrometry and mass photometry."],"pmcid":["PMC11999443"],"funding_grant_id":["SPI.2017.028"],"pubmed_authors":["Heck AJR","Barnes LF","Powers TW","Friese OV","Desligniere E"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of intact mRNA-based therapeutics by charge detection mass spectrometry and mass photometry.","description":"The impressive success of mRNA-based vaccines to combat COVID-19 has encouraged biopharmaceutical companies to invest in broader applications of alike vaccines for various diseases. Analytical approaches must keep pace to support this surge in the development of mRNA-based therapies. Intact mass analysis of mid- to large mRNA molecules (>1,000 nt) poses significant analytical challenges due to mRNA size, heterogeneity, and instability. Here, we demonstrate how single-particle Orbitrap-based charge detection mass spectrometry (CDMS) and mass photometry (MP) approaches can rapidly measure the mass of various intact high-mass capped mRNAs, up to 9,400 nt (∼3 MDa) in size. While ensemble MS yielded approximate masses for mRNAs <2,000 nt, it failed to provide information on samples of longer se","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2025-07-07T03:10:13.694Z","creation":"2025-07-07T03:10:13.694Z"},"accession":"S-EPMC11999443","cross_references":{"pubmed":["40236497"],"doi":["10.1016/j.omtm.2025.101454"]}}