biostudies-literatureUnknown49(4)Lambert C<h4>Background</h4>This study aims to investigate the differential miRNA expression profile between the visceral white adipose tissue and the skeletal muscle of people with obesity undergoing bariatric surgery.<h4>Methods</h4>Skeletal muscle and visceral adipose tissue samples of 10 controls and 38 people with obesity (50% also with type 2 diabetes) undergoing bariatric surgery were collected. miRNA expression profiles were analyzed using Next-Generation Sequencing and subsequently validated using RT-PCR.<h4>Results</h4>Approximately 69% of miRNAs showed similar expression in both tissues, however, 55 miRNAs were preferentially expressed in visceral adipose tissue and 53 in skeletal muscle. miR-122b-5p was uniquely identified in skeletal muscle, while miR-1-3p and miR-206 were upregulated in skeletal muscle. Conversely, miR-224-5p and miR-335-3p exhibited upregulation in visceral adipose tissue. Notably, distinctions related to the presence of type 2 diabetes were observed solely in the expression of miR-1-3p and miR-206 in visceral adipose tissue.<h4>Conclusions</h4>This is the first study unveiling distinct miRNA expression profiles in paired samples of visceral adipose tissue and skeletal muscle in humans. The identification of obesity-specific miRNAs in these tissues opens up promising avenues for research into potential biomarkers for obesity diagnosis and treatment.International journal of obesity (2005)634-641https://www.ebi.ac.uk/biostudies/studies/S-EPMC11999863biostudies-literatureExploring differential miRNA expression profiles in muscular and visceral adipose tissue of patients with severe obesity.PMC11999863Sanz Alvarez LMGarcia-Villarino MLatorre JGarcia AVUria RRMorales-Sanchez PMoreno Gijon MLambert CAres-Blanco JMenendez-Torre EVilla-Fernandez EFernandez-Real JMNavarro SSPujante PSuarez-Gutierrez LDelgado ETurienzo Santos EOfalseExploring differential miRNA expression profiles in muscular and visceral adipose tissue of patients with severe obesity.<h4>Background</h4>This study aims to investigate the differential miRNA expression profile between the visceral white adipose tissue and the skeletal muscle of people with obesity undergoing bariatric surgery.<h4>Methods</h4>Skeletal muscle and visceral adipose tissue samples of 10 controls and 38 people with obesity (50% also with type 2 diabetes) undergoing bariatric surgery were collected. miRNA expression profiles were analyzed using Next-Generation Sequencing and subsequently validated using RT-PCR.<h4>Results</h4>Approximately 69% of miRNAs showed similar expression in both tissues, however, 55 miRNAs were preferentially expressed in visceral adipose tissue and 53 in skeletal muscle. miR-122b-5p was uniquely identified in skeletal muscle, while miR-1-3p and miR-206 were upregulated in skeletal muscle. Conversely, miR-224-5p and miR-335-3p exhibited upregulation in visceral adipose tissue. Notably, distinctions related to the presence of type 2 diabetes were observed solely in the expression of miR-1-3p and miR-206 in visceral adipose tissue.<h4>Conclusions</h4>This is the first study unveiling distinct miRNA expression profiles in paired samples of visceral adipose tissue and skeletal muscle in humans. The identification of obesity-specific miRNAs in these tissues opens up promising avenues for research into potential biomarkers for obesity diagnosis and treatment.2025-01-01T00:00:00Z2025 Apr2026-06-01T20:42:18.383Z2025-07-03T03:06:51.398ZS-EPMC119998633956268710.1038/s41366-024-01683-4