biostudies-literatureAli AChinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science CenterProject of National Key Laboratory for Tropical Crop BreedingProject of Sanya Science and Technology InnovationJoint Special Project of Basic Agricultural Research of Yunnan ProvinceNational Key Research and Development Program of ChinaGuangxi Science and Technology Major Program484https://www.ebi.ac.uk/biostudies/studies/S-EPMC12001561biostudies-literatureUnknown25(1)<h4>Background</h4>The MAP Kinase cascade system is a conserved signaling mechanism essential for plant development, growth, and stress tolerance. Thus far, genes from the MAPK cascade have been identified in several plant species but remain uncharacterized in the polyploid Saccharum spp. Hybrid R570 genome.<h4>Results</h4>This study identified 89 ScMAPK, 24 ScMAPKK, and 107 ScMAPKKK genes through genome-wide analysis. Phylogenetic classification revealed that four subgroups were present in each ScMAPK and ScMAPKK family, and three sub-families (ZIK-like, RAF-like, and MEKK-like) presented in the ScMAPKKK family. Conserved motif and gene structure analysis supported the evolutionary relationships of the three families inferred from the phylogenetic analysis. All of the ScMAPK, ScMAPKK and BMC plant biologyGenome-wide identification and unveiling the role of MAP kinase cascade genes involved in sugarcane response to abiotic stressors.PMC12001561CATASCXTD2023072022YFD2301102NKLTCBCXTD23，NKLTCB-ZX03GuikeAA23073001-1-19202101BD070001-025No.2022KJCX17Zhao XTLin JSWu RJZhao TTAli AFeng CLHuang QXWang JGLi LLiu HBfalseGenome-wide identification and unveiling the role of MAP kinase cascade genes involved in sugarcane response to abiotic stressors.<h4>Background</h4>The MAP Kinase cascade system is a conserved signaling mechanism essential for plant development, growth, and stress tolerance. Thus far, genes from the MAPK cascade have been identified in several plant species but remain uncharacterized in the polyploid Saccharum spp. Hybrid R570 genome.<h4>Results</h4>This study identified 89 ScMAPK, 24 ScMAPKK, and 107 ScMAPKKK genes through genome-wide analysis. Phylogenetic classification revealed that four subgroups were present in each ScMAPK and ScMAPKK family, and three sub-families (ZIK-like, RAF-like, and MEKK-like) presented in the ScMAPKKK family. Conserved motif and gene structure analysis supported the evolutionary relationships of the three families inferred from the phylogenetic analysis. All of the ScMAPK, ScMAPKK and 2025-01-01T00:00:00Z2025 Apr2026-04-08T18:35:19.511Z2026-04-08T09:41:37.785ZS-EPMC120015614024095810.1186/s12870-025-06490-1