biostudies-literatureSantambrogio CMinistero dell'Università e della RicercaMinistero dell'Università e della Ricercae202500106https://www.ebi.ac.uk/biostudies/studies/S-EPMC12002116biostudies-literatureUnknown26(7)Recognition of glycans by simple synthetic receptors is a key issue in supramolecular chemistry, endowed with relevant implications in glycobiology and medicine. In this context, glycoproteins featuring N-glycans represent an important biological target, because they are often exploited by enveloped viruses in adhesion and infection processes. However, a direct evidence for their recognition by a synthetic receptor targeting N-glycans is still missing in the literature. Using a combination of glycoengineering and mass spectrometry techniques, we present here the direct evidence of biomimetic recognition of complex-type N-glycans exposed on the receptor-binding domain (RBD) of the wild-type spike protein of SARS-CoV-2 by a biologically active, synthetic receptor.Chembiochem : a European journal of chemical biologyBiomimetic Recognition of SARS-CoV-2 Receptor-Binding Domain N-Glycans by an Antiviral Synthetic Receptor.PMC12002116FISR2020IP_01095PE00000007, INF-ACTGrandori RPonzini ERoelens SSantambrogio CPesce EFragai MFrancesconi ODe Francesco RToccafondi MNativi CDonnici LMilanesi FGrifantini RfalseBiomimetic Recognition of SARS-CoV-2 Receptor-Binding Domain N-Glycans by an Antiviral Synthetic Receptor.Recognition of glycans by simple synthetic receptors is a key issue in supramolecular chemistry, endowed with relevant implications in glycobiology and medicine. In this context, glycoproteins featuring N-glycans represent an important biological target, because they are often exploited by enveloped viruses in adhesion and infection processes. However, a direct evidence for their recognition by a synthetic receptor targeting N-glycans is still missing in the literature. Using a combination of glycoengineering and mass spectrometry techniques, we present here the direct evidence of biomimetic recognition of complex-type N-glycans exposed on the receptor-binding domain (RBD) of the wild-type spike protein of SARS-CoV-2 by a biologically active, synthetic receptor.2025-01-01T00:00:00Z2025 Apr2025-07-08T03:11:00.615Z2025-07-08T03:11:00.615ZS-EPMC120021163998266110.1002/cbic.202500106