biostudies-literatureUnknown16(1)Wang Y<h4>Background</h4>Coagulation factor V (FV) is an essential cofactor in the coagulation cascade. However, the precise function of FV in lower grade glioma (LGG) is little known.<h4>Methods</h4>We first performed a pan-cancer investigation of FV expression and prognosis using TCGA and GTEx databases. Single-cell RNA sequencing confirmed FV expression in LGG tissues. We then investigated the mRNA expression level, prognostic value, and DNA methylation of FV in LGG using bioinformatics tools. The relationship between FV expression and tumor immune invasion was investigated using TIMER.<h4>Results</h4>FV was highly expressed in a variety of tumors, including LGG, and was associated with tumor prognosis. By combining a series of in silico analysis (including expression and survival analysis), Discover oncology535https://www.ebi.ac.uk/biostudies/studies/S-EPMC12003246biostudies-literatureClinical significance and immune infiltration analyses of the coagulation factor V gene in brain lower grade glioma.PMC12003246Deng CLi ZLi SZhang HGuo SWang YZhan YfalseClinical significance and immune infiltration analyses of the coagulation factor V gene in brain lower grade glioma.<h4>Background</h4>Coagulation factor V (FV) is an essential cofactor in the coagulation cascade. However, the precise function of FV in lower grade glioma (LGG) is little known.<h4>Methods</h4>We first performed a pan-cancer investigation of FV expression and prognosis using TCGA and GTEx databases. Single-cell RNA sequencing confirmed FV expression in LGG tissues. We then investigated the mRNA expression level, prognostic value, and DNA methylation of FV in LGG using bioinformatics tools. The relationship between FV expression and tumor immune invasion was investigated using TIMER.<h4>Results</h4>FV was highly expressed in a variety of tumors, including LGG, and was associated with tumor prognosis. By combining a series of in silico analysis (including expression and survival analysis), 2025-01-01T00:00:00Z2025 Apr2025-07-03T03:04:45.981Z2025-07-03T03:04:45.981ZS-EPMC120032464023793110.1007/s12672-025-02124-y