biostudies-literatureUnknown36(4)Clark-Cotton MRSuccessful cell division requires faithful division and segregation of organelles into daughter cells. The unicellular alga <i>Chlamydomonas reinhardtii</i> has a single, large chloroplast whose division is spatiotemporally coordinated with furrowing. Cytoskeletal structures form in the same plane at the midzone of the dividing chloroplast (FtsZ) and the cell (microtubules), but how these structures are coordinated is not understood. Previous work showed that loss of F-actin blocks chloroplast division but not furrow ingression, suggesting that pharmacological perturbations can disorganize these events. In this study, we developed an imaging platform to screen natural compounds that perturb cell division while monitoring FtsZ and microtubules and identified 70 unique compounds. One compounMolecular biology of the cellbr14https://www.ebi.ac.uk/biostudies/studies/S-EPMC12005104biostudies-literatureImaging-based screen identifies novel natural compounds that perturb cell and chloroplast division in &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt;.PMC12005104Clark-Cotton MRMalhotra VMulabagal AJChen SAOnishi MPerry AGomez AfalseImaging-based screen identifies novel natural compounds that perturb cell and chloroplast division in &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt;.Successful cell division requires faithful division and segregation of organelles into daughter cells. The unicellular alga <i>Chlamydomonas reinhardtii</i> has a single, large chloroplast whose division is spatiotemporally coordinated with furrowing. Cytoskeletal structures form in the same plane at the midzone of the dividing chloroplast (FtsZ) and the cell (microtubules), but how these structures are coordinated is not understood. Previous work showed that loss of F-actin blocks chloroplast division but not furrow ingression, suggesting that pharmacological perturbations can disorganize these events. In this study, we developed an imaging platform to screen natural compounds that perturb cell division while monitoring FtsZ and microtubules and identified 70 unique compounds. One compoun2025-01-01T00:00:00Z2025 Apr2026-05-29T16:34:48.396Z2026-04-08T05:25:38.698ZS-EPMC120051044002017910.1091/mbc.E24-09-0425