{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Qu X"],"funding":["Basic Research Program of Suzhou","Shandong Provincial Laboratory","Natural Science Foundation of Jiangsu Province"],"pagination":["6253-6259"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12007104"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(15)"],"pubmed_abstract":["Protein function investigation and clinical assay are fundamental to modern biology and medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication and repair, plays a critical role in the progression of many diseases. Taking FEN1 as an example, we present a novel protein detection platform combining triboelectric nanogenerator (TENG) and CRISPR/Cas technologies. As a specific form of TENG, the transistor-droplet electricity generator (TDEG) is explored, which offers a low-cost, simple fabrication approach with real-time detection capability. Meanwhile, the FEN1 activated CRISPR/Cas system catalyzes the reactions on the three-dimensional DNA tetrahedron interface, promising the high sensitivity. This work not only demonstrates a powerful method for rapid protein detect"],"journal":["Nano letters"],"pubmed_title":["Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification."],"pmcid":["PMC12007104"],"funding_grant_id":["BK20220048","SSD2024016","SYS202207"],"pubmed_authors":["Miao P","Qu X","Jiao J","Yue Y","Tang Y"],"additional_accession":[]},"is_claimable":false,"name":"Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification.","description":"Protein function investigation and clinical assay are fundamental to modern biology and medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication and repair, plays a critical role in the progression of many diseases. Taking FEN1 as an example, we present a novel protein detection platform combining triboelectric nanogenerator (TENG) and CRISPR/Cas technologies. As a specific form of TENG, the transistor-droplet electricity generator (TDEG) is explored, which offers a low-cost, simple fabrication approach with real-time detection capability. Meanwhile, the FEN1 activated CRISPR/Cas system catalyzes the reactions on the three-dimensional DNA tetrahedron interface, promising the high sensitivity. This work not only demonstrates a powerful method for rapid protein detect","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-12T03:04:23.376Z","creation":"2025-07-12T03:04:23.376Z"},"accession":"S-EPMC12007104","cross_references":{"pubmed":["40191992"],"doi":["10.1021/acs.nanolett.5c00877"]}}