biostudies-literatureSun HNatural Science Foundation of Shandong ProvinceScience and Technology Development Plan of the Shandong Administration of Traditional Chinese Medicine program13411https://www.ebi.ac.uk/biostudies/studies/S-EPMC12008290biostudies-literatureUnknown15(1)Previous studies conducted by the same group of researchers found that Traditional Chinese Medicine Astragalus mongholicus Bunge and Hedyotis diffusa Willd (A-H) significantly suppressed the cell proliferation of lung adenocarcinoma (LUAD). MicroRNAs are considered promising candidates for cancer diagnosis and treatment. This study focused on miR-582-3p as the primary subject of investigation to explore the mechanism by which A-H inhibits cell proliferation through miR-582-3p. The overexpressing and silencing miR-582-3p cell models were established by using lentiviral transfection technology. CCK-8 assay (24 h, 48 h, 72 h) and clone formation assay (1 w) were employed to detect the proliferation of A549 cells. Moreover, flow cytometry analysis (24 h) was performed to detect the cell cycle.Scientific reportsAstragalus mongholicus and Hedyotis diffusa willd inhibit cell proliferation by attenuating the miR-582-3p-p27 signaling pathway in LUAD.PMC12008290ZR2023QH0722021Q076Xu LLiu SSun HMa TfalseAstragalus mongholicus and Hedyotis diffusa willd inhibit cell proliferation by attenuating the miR-582-3p-p27 signaling pathway in LUAD.Previous studies conducted by the same group of researchers found that Traditional Chinese Medicine Astragalus mongholicus Bunge and Hedyotis diffusa Willd (A-H) significantly suppressed the cell proliferation of lung adenocarcinoma (LUAD). MicroRNAs are considered promising candidates for cancer diagnosis and treatment. This study focused on miR-582-3p as the primary subject of investigation to explore the mechanism by which A-H inhibits cell proliferation through miR-582-3p. The overexpressing and silencing miR-582-3p cell models were established by using lentiviral transfection technology. CCK-8 assay (24 h, 48 h, 72 h) and clone formation assay (1 w) were employed to detect the proliferation of A549 cells. Moreover, flow cytometry analysis (24 h) was performed to detect the cell cycle.2025-01-01T00:00:00Z2025 Apr2025-07-03T03:04:48.108Z2025-07-03T03:04:48.108ZS-EPMC120082904025129210.1038/s41598-025-97996-z