{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["Jilin Provincial Department of Science and Technology","Changchun University Climbing Program"],"pagination":["13440"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12008293"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient is a polysaccharide, which has a prominent immunomodulatory effect. In this study, the purified polysaccharide component of Acanthopanax senticosus leaves(ASPS-A1) with strong immunomodulatory activity was isolated by column chromatography, and its structure and properties were characterized by HPGPC, and FT-IR. Results showed that ASPS-A1 is mainly composed of α- 1,4-<sub>D</sub>-GalA, α- 1,5-<sub>L</sub>-Ara, and β- 1,4-<sub>D</sub>-Gal. RT-qPCR experiments and RNA-seq analysis were used to study the immunoregulatory mechanism of ASPS-A1. The results showed that ASPS-A1 could significantly up-regulate the levels of cytokines iNOS, IL- 1β, IL- 6, and TNF-α activated macrophages through MAPK, NF-κB, and Toll-like receptor signaling pathways. Inhibitory experiments further confirmed that ASPS-A1 promotes the expression of iNOS, TNF-α, and IL- 6 via the TLR4 receptor, and TNF-α and IL- 1β via the TLR2 receptor. In order to identify the target of ASPS-A1, molecular docking experiments were conducted. The results demonstrated that ASPS-A1 could bind to both TLR4 and TLR2, forming stable complexes with the cavities on the protein surface through hydrogen bonding and hydrophobic interaction. The docking scores indicated that ASPS-A1 could regulate the immune response through TLR2 and TLR4 signaling pathways, with a particularly strong interaction with TLR4. In summary, this study screened and characterized the most immunoreactive components of Acanthopanax senticosus polysaccharide, disclosed the immunomodulatory mechanism of ASPS-A1, and furnished a research basis for its potential application as a natural immune enhancer."],"journal":["Scientific reports"],"pubmed_title":["Immunostimulatory effects mechanism of polysaccharide extracted from Acanthopanax senticosus on RAW 264.7 cells through activating the TLR/MAPK/NF-κB signaling pathway."],"pmcid":["PMC12008293"],"funding_grant_id":["YDZJ202101ZYTS077","ZPK202123"],"pubmed_authors":["Liu J","Zhang H","Ren P","Hong F","Wang Y","Cheng X","Zhang R","Zhao J","Gou D","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Immunostimulatory effects mechanism of polysaccharide extracted from Acanthopanax senticosus on RAW 264.7 cells through activating the TLR/MAPK/NF-κB signaling pathway.","description":"Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient is a polysaccharide, which has a prominent immunomodulatory effect. In this study, the purified polysaccharide component of Acanthopanax senticosus leaves(ASPS-A1) with strong immunomodulatory activity was isolated by column chromatography, and its structure and properties were characterized by HPGPC, and FT-IR. Results showed that ASPS-A1 is mainly composed of α- 1,4-<sub>D</sub>-GalA, α- 1,5-<sub>L</sub>-Ara, and β- 1,4-<sub>D</sub>-Gal. RT-qPCR experiments and RNA-seq analysis were used to study the immunoregulatory mechanism of ASPS-A1. The results showed that ASPS-A1 could significantly up-regulate the levels of cytokines iNOS, IL- 1β, IL- 6, and TNF-α activated macrophages through MAPK, NF-κB, and Toll-like receptor signaling pathways. Inhibitory experiments further confirmed that ASPS-A1 promotes the expression of iNOS, TNF-α, and IL- 6 via the TLR4 receptor, and TNF-α and IL- 1β via the TLR2 receptor. In order to identify the target of ASPS-A1, molecular docking experiments were conducted. The results demonstrated that ASPS-A1 could bind to both TLR4 and TLR2, forming stable complexes with the cavities on the protein surface through hydrogen bonding and hydrophobic interaction. The docking scores indicated that ASPS-A1 could regulate the immune response through TLR2 and TLR4 signaling pathways, with a particularly strong interaction with TLR4. In summary, this study screened and characterized the most immunoreactive components of Acanthopanax senticosus polysaccharide, disclosed the immunomodulatory mechanism of ASPS-A1, and furnished a research basis for its potential application as a natural immune enhancer.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-14T03:02:43.471Z","creation":"2025-07-14T03:02:43.471Z"},"accession":"S-EPMC12008293","cross_references":{"pubmed":["40251266"],"doi":["10.1038/s41598-025-97423-3"]}}