biostudies-literatureJeong YJYonsei University College of MedicineCommercializations Promotion Agency for R and D OutcomesMinistry of Science and ICT, South Korea187https://www.ebi.ac.uk/biostudies/studies/S-EPMC12008940biostudies-literatureUnknown16(1)<h4>Background</h4>Salivary gland (SG) hypofunction presents a significant clinical challenge with limited treatment options. SG epithelial cells offer a promising approach due to their intrinsic tissue specificity and regenerative potential. However, the lack of efficient culture methods has hindered their clinical use.<h4>Methods</h4>This study presents a chemical reprogramming culture (CRC) system that utilizes a combination of three small molecules for the long-term two-dimensional culture of human SG epithelial progenitor cells. We characterized the cultured cells, measured their organoid-forming efficiencies, and assessed their differentiation potential. To evaluate the therapeutic efficacy of the SG basal progenitor cells (SG-BPCs), we administered them into a mouse model with radiaStem cell research & therapyChemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells.PMC12008940RS-2022-001648786-2022-0106RS-2024-00432946Hong SMHong YSim NSLim JYJeong YJYoon YJfalseChemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells.<h4>Background</h4>Salivary gland (SG) hypofunction presents a significant clinical challenge with limited treatment options. SG epithelial cells offer a promising approach due to their intrinsic tissue specificity and regenerative potential. However, the lack of efficient culture methods has hindered their clinical use.<h4>Methods</h4>This study presents a chemical reprogramming culture (CRC) system that utilizes a combination of three small molecules for the long-term two-dimensional culture of human SG epithelial progenitor cells. We characterized the cultured cells, measured their organoid-forming efficiencies, and assessed their differentiation potential. To evaluate the therapeutic efficacy of the SG basal progenitor cells (SG-BPCs), we administered them into a mouse model with radia2025-01-01T00:00:00Z2025 Apr2025-06-28T03:05:34.844Z2025-06-28T03:05:34.844ZS-EPMC120089404025160110.1186/s13287-025-04295-5