biostudies-literatureUnknown16Hu X<h4>Aim</h4>To investigate the effect and mechanism of Sitagliptin phosphate on inflammation and macrophage polarization in a mouse model of type 2 diabetes.<h4>Methods</h4><i>In vitro</i>, Raw264.7 cells were cultured with a high concentration of glucose (HG) and sitagliptin phosphate (SIG). The levels of inflammatory factors and the regulation of macrophage polarization were investigated, and the differentially expressed genes between HG and HG+SIG intervention were analyzed and enriched through transcriptomics. <i>In vivo</i>, C57BL/6J male mice were treated with HFD+STZ to establish a type 2 diabetes mouse model were investigated the effects of regulation of macrophage polarization in the pancreas and visceral adipose tissue.<h4>Results</h4>In vitro cell experiments and transcriptomicsFrontiers in endocrinology1544684https://www.ebi.ac.uk/biostudies/studies/S-EPMC12010097biostudies-literatureSitagliptin phosphate ameliorates chronic inflammation in diabetes mellitus via modulating macrophage polarization.PMC12010097Liu XLi YHu XfalseSitagliptin phosphate ameliorates chronic inflammation in diabetes mellitus via modulating macrophage polarization.<h4>Aim</h4>To investigate the effect and mechanism of Sitagliptin phosphate on inflammation and macrophage polarization in a mouse model of type 2 diabetes.<h4>Methods</h4><i>In vitro</i>, Raw264.7 cells were cultured with a high concentration of glucose (HG) and sitagliptin phosphate (SIG). The levels of inflammatory factors and the regulation of macrophage polarization were investigated, and the differentially expressed genes between HG and HG+SIG intervention were analyzed and enriched through transcriptomics. <i>In vivo</i>, C57BL/6J male mice were treated with HFD+STZ to establish a type 2 diabetes mouse model were investigated the effects of regulation of macrophage polarization in the pancreas and visceral adipose tissue.<h4>Results</h4>In vitro cell experiments and transcriptomics2025-01-01T00:00:00Z20252026-05-29T14:48:11.482Z2025-07-03T03:04:57.479ZS-EPMC120100974026028310.3389/fendo.2025.1544684