biostudies-literatureUnknown18(4)Prabin KRe-utilising brewers' spent grain (BSG) through LAB fermentation can enable its broad use in the food industry, enhancing its nutritional and functional properties and offering a clear example of a sustainable approach in the valorisation of food side streams. Despite extensive research on LAB fermentation, the regulation of metabolism during the growth in complex food-industry-relevant environments remains unclear. This study investigates the metabolic processes in Leuconostoc pseudomesenteroides DSM20193 during 24 h fermentation of BSG with and without 4% sucrose (w/w) supplementation, allowing in situ dextran synthesis. Besides dextran synthesis, the presence of sucrose led to faster acidification, especially due to the increased formation of acetic acid. Furthermore, differences in theMicrobial biotechnologye70116https://www.ebi.ac.uk/biostudies/studies/S-EPMC12010140biostudies-literatureRegulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193.PMC12010140Prabin KDominik MNdegwa MRossana CfalseRegulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193.Re-utilising brewers' spent grain (BSG) through LAB fermentation can enable its broad use in the food industry, enhancing its nutritional and functional properties and offering a clear example of a sustainable approach in the valorisation of food side streams. Despite extensive research on LAB fermentation, the regulation of metabolism during the growth in complex food-industry-relevant environments remains unclear. This study investigates the metabolic processes in Leuconostoc pseudomesenteroides DSM20193 during 24 h fermentation of BSG with and without 4% sucrose (w/w) supplementation, allowing in situ dextran synthesis. Besides dextran synthesis, the presence of sucrose led to faster acidification, especially due to the increased formation of acetic acid. Furthermore, differences in the2025-01-01T00:00:00Z2025 Apr2025-07-07T03:10:07.622Z2025-07-07T03:10:07.622ZS-EPMC120101404025685910.1111/1751-7915.70116