biostudies-literatureWeeratunga PCornell Feline Health Center195https://www.ebi.ac.uk/biostudies/studies/S-EPMC12010622biostudies-literatureUnknown16(1)<h4>Background</h4>Induced pluripotent stem cells (iPSCs) can propagate indefinitely and give rise to every other cell type, rendering them invaluable for disease modelling, drug development research, and usage in regenerative medicine. While feline iPSCs have been described, there are currently no reports on generating genome integration (footprint)-free iPSCs from domestic cats. Therefore, the objective of this study was to generate feline iPSCs from fetal fibroblasts using non-integrative Sendai virus (SeV) vectors carrying human transcription factors. Moreover, these iPSCs were differentiated into mesenchymal stromal cells (MSCs), which can be used as an alternative to tissue-derived MSCs.<h4>Methods</h4>Feline fetal fibroblasts were transduced with CytoTune-iPS 2.0 Sendai ReprogramminStem cell research & therapyFootprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model.PMC12010622NAJager MCWeeratunga PHarman RMVan de Walle GRfalseFootprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model.<h4>Background</h4>Induced pluripotent stem cells (iPSCs) can propagate indefinitely and give rise to every other cell type, rendering them invaluable for disease modelling, drug development research, and usage in regenerative medicine. While feline iPSCs have been described, there are currently no reports on generating genome integration (footprint)-free iPSCs from domestic cats. Therefore, the objective of this study was to generate feline iPSCs from fetal fibroblasts using non-integrative Sendai virus (SeV) vectors carrying human transcription factors. Moreover, these iPSCs were differentiated into mesenchymal stromal cells (MSCs), which can be used as an alternative to tissue-derived MSCs.<h4>Methods</h4>Feline fetal fibroblasts were transduced with CytoTune-iPS 2.0 Sendai Reprogrammin2025-01-01T00:00:00Z2025 Apr2026-06-01T13:05:35.573Z2025-07-03T03:05:05.266ZS-EPMC120106224025456910.1186/s13287-025-04325-2