biostudies-literatureUnknown16Liu R<h4>Introduction</h4>Ammonium (NH₄ ⁺) and nitrate (NO₃ ^-) are the two main forms of inorganic nitrogen (N) that exist in soil and both can be absorbed and utilized by plants. As a vast and crucial biome, soil microorganisms are responsible for mediating the inorganic N assimilation process and enhancing nitrogen use efficiency. Understanding how these microorganisms assimilate different forms of inorganic nitrogen is crucial. There are a handful of microorganisms that play a dominant role in the process of soil inorganic nitrogen assimilation and have a significant advantage in abundance. However, microbial preferences for ammonium or nitrate, as well as differences in their metabolic pathways under co-existing ammonium and nitrate conditions, reFrontiers in microbiology1559884https://www.ebi.ac.uk/biostudies/studies/S-EPMC12010642biostudies-literatureTranscriptome analysis of nitrogen assimilation preferences in &lt;i&gt;Burkholderia&lt;/i&gt; sp. M6-3 and &lt;i&gt;Arthrobacter&lt;/i&gt; sp. M7-15.PMC12010642Jiang YChu CWang QQin HZhong WLiu RHan CDeng HfalseTranscriptome analysis of nitrogen assimilation preferences in &lt;i&gt;Burkholderia&lt;/i&gt; sp. M6-3 and &lt;i&gt;Arthrobacter&lt;/i&gt; sp. M7-15.<h4>Introduction</h4>Ammonium (NH₄ ⁺) and nitrate (NO₃ ^-) are the two main forms of inorganic nitrogen (N) that exist in soil and both can be absorbed and utilized by plants. As a vast and crucial biome, soil microorganisms are responsible for mediating the inorganic N assimilation process and enhancing nitrogen use efficiency. Understanding how these microorganisms assimilate different forms of inorganic nitrogen is crucial. There are a handful of microorganisms that play a dominant role in the process of soil inorganic nitrogen assimilation and have a significant advantage in abundance. However, microbial preferences for ammonium or nitrate, as well as differences in their metabolic pathways under co-existing ammonium and nitrate conditions, re2025-01-01T00:00:00Z20252026-06-01T21:07:47.339Z2025-07-03T03:05:12.999ZS-EPMC120106424026008810.3389/fmicb.2025.1559884