{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Zhu P"],"pubmed_abstract":["Toxocariasis, caused by the dog roundworm <i>Toxocara canis,</i> is a globally distributed zoonotic parasitic disease that poses a significant threat to veterinary and public health. The ubiquitin plus an L40 ribosomal protein (UBQ-2) in parasites plays a crucial role in protein degradation and meiotic divisions, thereby affecting parasite development, survival, and parasite-host interactions. In this study, we identified for the first time a full-length cDNA encoding the UBQ-2 protein from the <i>T. canis</i>-omic dataset, called Tcubq-2. After cloning and sequencing, we conducted sequence analysis and structural modeling of TcUBQ-2 using online bioinformatics tools. The recombinant TcUBQ-2 protein (rTcUBQ-2) was prokaryotically expressed and subjected to Western blot analysis to assess i"],"journal":["Frontiers in veterinary science"],"pagination":["1550489"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12010969"],"repository":["biostudies-literature"],"pubmed_title":["Cloning, bioinformatics analysis, and expression of the ubiquitin 2 (ubq-2) gene from the dog roundworm &lt;i&gt;Toxocara canis&lt;/i&gt;."],"pmcid":["PMC12010969"],"pubmed_authors":["Fan X","Xie Y","Huang J","Hu X","Wang H","He Q","Zhou X","Wang L","Zhu P","Li Z","Wang R","Wei G","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"Cloning, bioinformatics analysis, and expression of the ubiquitin 2 (ubq-2) gene from the dog roundworm &lt;i&gt;Toxocara canis&lt;/i&gt;.","description":"Toxocariasis, caused by the dog roundworm <i>Toxocara canis,</i> is a globally distributed zoonotic parasitic disease that poses a significant threat to veterinary and public health. The ubiquitin plus an L40 ribosomal protein (UBQ-2) in parasites plays a crucial role in protein degradation and meiotic divisions, thereby affecting parasite development, survival, and parasite-host interactions. In this study, we identified for the first time a full-length cDNA encoding the UBQ-2 protein from the <i>T. canis</i>-omic dataset, called Tcubq-2. After cloning and sequencing, we conducted sequence analysis and structural modeling of TcUBQ-2 using online bioinformatics tools. The recombinant TcUBQ-2 protein (rTcUBQ-2) was prokaryotically expressed and subjected to Western blot analysis to assess i","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-05-28T03:12:04.282Z","creation":"2025-07-03T03:05:24.045Z"},"accession":"S-EPMC12010969","cross_references":{"pubmed":["40260211"],"doi":["10.3389/fvets.2025.1550489"]}}