{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li M"],"funding":["MOST | National Key Research and Development Program of China (NKPs)","MOST | National Natural Science Foundation of China (NSFC)","MOST | National Key Research and Development Program of China","MOST | National Natural Science Foundation of China"],"pagination":["e2414731122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12012489"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(15)"],"pubmed_abstract":["The tubulin tail is a key element for microtubule (MT) functionality, but the functional redundancy of tubulin genes complicates the genetic determination of their physiological functions. Here, we removed the C-terminal tail of five alpha- and four beta-tubulin genes in the <i>C. elegans</i> genome. Sensory cilia typically exhibit an axoneme that longitudinally differentiates into a middle segment with doublet MTs and a distal segment with singlet MTs. However, the excision of the alpha-tubulin tail, but not the beta-tubulin tail, resulted in the ectopic formation of doublet MTs in the distal segments. Molecular dynamics simulations suggest that the alpha-tubulin tail could prevent the B-tubule from docking on the surface of A-tubule. Using recombinant tubulins, we demonstrated that remov"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Alpha-tubulin tails regulate axoneme differentiation."],"pmcid":["PMC12012489"],"funding_grant_id":["92254306","2022YFA1302700","32270773","32270721","32070706","32470730","31991190","2019YFA0508401"],"pubmed_authors":["Li W","Guo Z","Chen Z","Ou G","Wang Y","Chai Y","Li M"],"additional_accession":[]},"is_claimable":false,"name":"Alpha-tubulin tails regulate axoneme differentiation.","description":"The tubulin tail is a key element for microtubule (MT) functionality, but the functional redundancy of tubulin genes complicates the genetic determination of their physiological functions. Here, we removed the C-terminal tail of five alpha- and four beta-tubulin genes in the <i>C. elegans</i> genome. Sensory cilia typically exhibit an axoneme that longitudinally differentiates into a middle segment with doublet MTs and a distal segment with singlet MTs. However, the excision of the alpha-tubulin tail, but not the beta-tubulin tail, resulted in the ectopic formation of doublet MTs in the distal segments. Molecular dynamics simulations suggest that the alpha-tubulin tail could prevent the B-tubule from docking on the surface of A-tubule. Using recombinant tubulins, we demonstrated that remov","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-15T07:34:00.634Z","creation":"2026-07-02T03:08:40.785Z"},"accession":"S-EPMC12012489","cross_references":{"pubmed":["40198703"],"doi":["10.1073/pnas.2414731122"]}}