{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ding Y"],"funding":["National Natural Science Grant of China","Natural Science Foundation of Shanghai","Pyramid Talent Project"],"pagination":["szaf009"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12012893"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(4)"],"pubmed_abstract":["<h4>Background</h4>Mesenchymal stem cells (MSCs) require priming by proinflammatory stimuli for optimal immunosuppressive effects. Our previous work identified mixed lymphocyte reaction-conditioned medium (MLR-CdM) as a potent enhancer of MSC immunosuppressive properties. This study evaluates the immunomodulatory potential of MSC-derived extracellular vesicles preconditioned with MLR-CdM (MSC-EVMLR) compared to IFN-γ (MSC-EVIFN), focusing on key miRNAs and mechanisms involved.<h4>Methods</h4>We assessed the ability of MSC-EVMLR and MSC-EVIFN to modulate lymphocyte proliferation and cytokine expression in vitro. To identify potential effector molecules within MSC-EVMLR, we performed miRNA array analysis combined with dose-response experiments using MLR-CdM under varying stimulation conditio"],"journal":["Stem cells translational medicine"],"pubmed_title":["Mixed lymphocyte reaction-conditioned MSC-derived extracellular vesicles enhance graft survival via miR-638-mediated immunoregulation."],"pmcid":["PMC12012893"],"funding_grant_id":["81500207","YQ677","24ZR1459300"],"pubmed_authors":["Ding Y","Zheng X","Liang X","Lin F","Zhu F","Chen Y","Ma K","Han S","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Mixed lymphocyte reaction-conditioned MSC-derived extracellular vesicles enhance graft survival via miR-638-mediated immunoregulation.","description":"<h4>Background</h4>Mesenchymal stem cells (MSCs) require priming by proinflammatory stimuli for optimal immunosuppressive effects. Our previous work identified mixed lymphocyte reaction-conditioned medium (MLR-CdM) as a potent enhancer of MSC immunosuppressive properties. This study evaluates the immunomodulatory potential of MSC-derived extracellular vesicles preconditioned with MLR-CdM (MSC-EVMLR) compared to IFN-γ (MSC-EVIFN), focusing on key miRNAs and mechanisms involved.<h4>Methods</h4>We assessed the ability of MSC-EVMLR and MSC-EVIFN to modulate lymphocyte proliferation and cytokine expression in vitro. To identify potential effector molecules within MSC-EVMLR, we performed miRNA array analysis combined with dose-response experiments using MLR-CdM under varying stimulation conditio","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-01T03:05:19.33Z","creation":"2025-07-01T03:05:19.33Z"},"accession":"S-EPMC12012893","cross_references":{"pubmed":["40261200"],"doi":["10.1093/stcltm/szaf009"]}}