{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Toribio D"],"funding":["NEI NIH HHS"],"pagination":["44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12013672"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["66(4)"],"pubmed_abstract":["Purpose
The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.Methods
LGs were isolated from an ADDE mouse model, male non-obese diabetic (NOD) mice and control wild-type BALB/c mice (n = 6 each). Protein samples were prepared in urea-based lysis buffer and protein concentrations determined by the BCA method. The equivalent of 200 µg protein were tryptically digested and analyzed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins were identified and "],"journal":["Investigative ophthalmology & visual science"],"pubmed_title":["Quantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjogren's Disease Animal Model."],"pmcid":["PMC12013672"],"funding_grant_id":["R01 EY029870"],"pubmed_authors":["Morokuma J","Pellino D","Zoukhri D","Toribio D","Hardt M"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjogren's Disease Animal Model.","description":"Purpose
The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.Methods
LGs were isolated from an ADDE mouse model, male non-obese diabetic (NOD) mice and control wild-type BALB/c mice (n = 6 each). Protein samples were prepared in urea-based lysis buffer and protein concentrations determined by the BCA method. The equivalent of 200 µg protein were tryptically digested and analyzed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins were identified and ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-06-03T01:27:26.674Z","creation":"2025-07-03T03:04:55.201Z"},"accession":"S-EPMC12013672","cross_references":{"pubmed":["40244610"],"doi":["10.1167/iovs.66.4.44"]}}