biostudies-literatureToribio DNEI NIH HHS44https://www.ebi.ac.uk/biostudies/studies/S-EPMC12013672biostudies-literatureUnknown66(4)<h4>Purpose</h4>The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.<h4>Methods</h4>LGs were isolated from an ADDE mouse model, male non-obese diabetic (NOD) mice and control wild-type BALB/c mice (n = 6 each). Protein samples were prepared in urea-based lysis buffer and protein concentrations determined by the BCA method. The equivalent of 200 µg protein were tryptically digested and analyzed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins were identified and Investigative ophthalmology & visual scienceQuantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjogren's Disease Animal Model.PMC12013672R01 EY029870Morokuma JPellino DZoukhri DToribio DHardt MfalseQuantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjogren's Disease Animal Model.<h4>Purpose</h4>The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.<h4>Methods</h4>LGs were isolated from an ADDE mouse model, male non-obese diabetic (NOD) mice and control wild-type BALB/c mice (n = 6 each). Protein samples were prepared in urea-based lysis buffer and protein concentrations determined by the BCA method. The equivalent of 200 µg protein were tryptically digested and analyzed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins were identified and 2025-01-01T00:00:00Z2025 Apr2026-06-03T01:27:26.674Z2025-07-03T03:04:55.201ZS-EPMC120136724024461010.1167/iovs.66.4.44