biostudies-literatureUnknown9(5)Steixner-Kumar AA<h4>Background</h4>Metabolic dysfunction-associated steatotic liver disease (MASLD) progresses to metabolic dysfunction-associated steatohepatitis (MASH) and is a major cause of liver cirrhosis. Although liver inflammation is the hallmark feature of MASH versus MASLD, the involvement of the peripheral immune cell compartments in disease progression is poorly understood, and single-cell profiles of peripheral immune cells in MASLD/MASH are not known.<h4>Methods</h4>Patients with MASLD/MASH and healthy volunteers have been prospectively enrolled in a cross-sectional study. Patients have been histologically stratified and further characterized by liver bulk RNA sequencing (RNA-Seq). Peripheral immune cells from patients and control blood samples have been comprehensively profiled using bulk aHepatology communicationse0643https://www.ebi.ac.uk/biostudies/studies/S-EPMC12014121biostudies-literatureSingle-cell landscape of peripheral immune cells in MASLD/MASH.PMC12014121Krenkel OBarth TFEHaenle MKratzer WWerner MBahrami EGeiger TSchlingeloff PRust WDick ASteixner-Kumar AASantacruz DSchmidberger JBottner DOkafo GSimon ENeubauer HfalseSingle-cell landscape of peripheral immune cells in MASLD/MASH.<h4>Background</h4>Metabolic dysfunction-associated steatotic liver disease (MASLD) progresses to metabolic dysfunction-associated steatohepatitis (MASH) and is a major cause of liver cirrhosis. Although liver inflammation is the hallmark feature of MASH versus MASLD, the involvement of the peripheral immune cell compartments in disease progression is poorly understood, and single-cell profiles of peripheral immune cells in MASLD/MASH are not known.<h4>Methods</h4>Patients with MASLD/MASH and healthy volunteers have been prospectively enrolled in a cross-sectional study. Patients have been histologically stratified and further characterized by liver bulk RNA sequencing (RNA-Seq). Peripheral immune cells from patients and control blood samples have been comprehensively profiled using bulk a2025-01-01T00:00:00Z2025 May2026-06-15T06:15:54.332Z2026-06-15T03:08:40.779ZS-EPMC120141214025730110.1097/HC9.0000000000000643