{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhu Z"],"funding":["Fundamental Research Funds for the Central Universities","National Natural Science Foundation of China","Huazhong University of Science and Technology"],"pagination":["gkaf311"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12014286"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(8)"],"pubmed_abstract":["The CRISPR-Cas12a system is widely used in nucleic acid detection and biosensing due to its high sensitivity, selectivity, and simple design. However, traditional CRISPR-Cas12a sensors, which rely on linear activators, face challenges such as limited operability and low stability. This study explored the impact of three different activator topologies-linear, planar, and steric-on the trans-cleavage activity of Cas12a. We developed a Cas12a-based switch using a planar activator, which demonstrated superior operability and maintained higher activity compared to linear activators. Using this planar activator, we achieved highly sensitive detection of hypochlorous acid, with a detection limit as low as 88 nM, outperforming chemical probe-based methods. The introduction of topological activators will open new avenues for the development of CRISPR-Cas12a-based biosensors, offering broad potential for diverse applications."],"journal":["Nucleic acids research"],"pubmed_title":["Exploring the effect of activator topology on CRISPR-Cas12a trans-cleavage activity."],"pmcid":["PMC12014286"],"funding_grant_id":["2024JYCXJJ010","82172372","22474045"],"pubmed_authors":["Li X","Zhu Z","Wu T","Ding L"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the effect of activator topology on CRISPR-Cas12a trans-cleavage activity.","description":"The CRISPR-Cas12a system is widely used in nucleic acid detection and biosensing due to its high sensitivity, selectivity, and simple design. However, traditional CRISPR-Cas12a sensors, which rely on linear activators, face challenges such as limited operability and low stability. This study explored the impact of three different activator topologies-linear, planar, and steric-on the trans-cleavage activity of Cas12a. We developed a Cas12a-based switch using a planar activator, which demonstrated superior operability and maintained higher activity compared to linear activators. Using this planar activator, we achieved highly sensitive detection of hypochlorous acid, with a detection limit as low as 88 nM, outperforming chemical probe-based methods. The introduction of topological activators will open new avenues for the development of CRISPR-Cas12a-based biosensors, offering broad potential for diverse applications.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-02T03:05:02.769Z","creation":"2025-07-02T03:05:02.769Z"},"accession":"S-EPMC12014286","cross_references":{"pubmed":["40263707"],"doi":["10.1093/nar/gkaf311"]}}