biostudies-literatureThapa PUSDA13https://www.ebi.ac.uk/biostudies/studies/S-EPMC12015871biostudies-literatureUnknown13(2)<h4>Background</h4>Tomatoes are self-pollinating plants, and successful fruit set depends on the production of functional pollen within the same flower. Our previous studies have shown that the 'Black Vernissage' tomato variety exhibits greater resilience to heat stress in terms of pollen productivity compared to the 'Micro-Tom' variety. Pollen productivity is determined by meiotic activity during microsporogenesis and the development of free microspores during gametogenesis. This study focused on identifying heat stress (HS)-induced proteomes in pollen mother cells (PMCs) and microspores.<h4>Methods</h4>Tomato plants were grown under two temperature conditions: 26 °C (non-heat-treated control) and 37 °C (heat-treated). Homogeneous cell samples of meiotic PMCs (prior to the tetrad stage) aProteomesCell-Type-Specific Heat-Induced Changes in the Proteomes of Pollen Mother Cells and Microspores Provide New Insights into Tomato Pollen Production Under Elevated Temperature.PMC12015871USDA-NIFA 2022-38821-37339; USDA-ARS CRIS Project 8062-211000-049Thapa PLi HZou JWang CZhou SPradhan KPotts JThapa DThannhauser TWO'Hair JYang YGuo JMadhavarapu SFish TfalseCell-Type-Specific Heat-Induced Changes in the Proteomes of Pollen Mother Cells and Microspores Provide New Insights into Tomato Pollen Production Under Elevated Temperature.<h4>Background</h4>Tomatoes are self-pollinating plants, and successful fruit set depends on the production of functional pollen within the same flower. Our previous studies have shown that the 'Black Vernissage' tomato variety exhibits greater resilience to heat stress in terms of pollen productivity compared to the 'Micro-Tom' variety. Pollen productivity is determined by meiotic activity during microsporogenesis and the development of free microspores during gametogenesis. This study focused on identifying heat stress (HS)-induced proteomes in pollen mother cells (PMCs) and microspores.<h4>Methods</h4>Tomato plants were grown under two temperature conditions: 26 °C (non-heat-treated control) and 37 °C (heat-treated). Homogeneous cell samples of meiotic PMCs (prior to the tetrad stage) a2025-01-01T00:00:00Z2025 Mar2025-07-02T03:05:06.771Z2025-07-02T03:05:06.771ZS-EPMC120158714026541810.3390/proteomes13020013