biostudies-literaturePaindelli CBayer HealthCare Pharmaceuticals IncNCI NIH HHSUniversity of Texas MD Anderson Cancer CenterNIH83-92https://www.ebi.ac.uk/biostudies/studies/S-EPMC12016311biostudies-literatureUnknown178Bone metastases are the most common milestone in the lethal progression of prostate cancer and prominent in a substantial portion of renal malignancies. Interactions between cancer and bone host cells have emerged as drivers of both disease progression and therapeutic resistance. To best understand these central host-epithelial cell interactions, biologically relevant preclinical models are required. To achieve this goal, we here established and characterized tissue-engineered bone mimetic environments (BME) capable of supporting the growth of patient-derived xenograft (PDX) cells, ex vivo and in vivo. The BME consisted of a polycaprolactone (PCL) scaffold colonized by human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. PDX-derived cells were isolated from bone metastaticActa biomaterialiaBone mimetic environments support engineering, propagation, and analysis of therapeutic response of patient-derived cells, ex vivo and in vivo.PMC12016311P30 CA016672P50 CA140388Barrios SNavone NWang WLMikos AGPan TDondossola EShepherd PLogothetis CJCampbell MTSatcher RLParietti VPaindelli CfalseBone mimetic environments support engineering, propagation, and analysis of therapeutic response of patient-derived cells, ex vivo and in vivo.Bone metastases are the most common milestone in the lethal progression of prostate cancer and prominent in a substantial portion of renal malignancies. Interactions between cancer and bone host cells have emerged as drivers of both disease progression and therapeutic resistance. To best understand these central host-epithelial cell interactions, biologically relevant preclinical models are required. To achieve this goal, we here established and characterized tissue-engineered bone mimetic environments (BME) capable of supporting the growth of patient-derived xenograft (PDX) cells, ex vivo and in vivo. The BME consisted of a polycaprolactone (PCL) scaffold colonized by human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. PDX-derived cells were isolated from bone metastatic2024-01-01T00:00:00Z2024 Apr2025-07-02T03:05:04.276Z2025-07-02T03:05:04.276ZS-EPMC120163113838774810.1016/j.actbio.2024.02.025